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大鼠皮质N |
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罗成义 徐如祥 王清华
摘 要 目的：观测N-甲基-D-天冬氨酸(NMDA)受体在脑损伤后的变化规律以及与继发性脑水肿发生和发展的关系。方法：用放射性配基结合分析法对伤后不同时间的大鼠伤侧大脑皮质NMDA受体活性进行测定；用干湿法测伤后伤侧皮质水含量。结果：NMDA受体活性(Bmax)于伤后30 min迅速上升达高峰，脑水肿于伤后6～24 h最明显；用NMDA受体的竞争性拮抗剂AP5(2-amino-5-phosphonlanoic acid)治疗后，脑水肿及NMDA受体活性与损伤组比较明显降低。结论：脑损伤后释放的谷氨酸可通过激活NMDA受体而发挥神经元兴奋毒作用。
主题词 脑损伤；脑水肿；大鼠
The changes of NMDA receptor activities in neuronal membrane after brain injury
LUO Cheng-Yi， XU Ru-Xiang， WANG Qing-Hua
Department of Neurosurgery, Zhujiang Hospital, The First Military Medical University, Guangzhou(510282)
Abstract AIM To study the changes of NMDA (N-methyl-D-aspartate) receptor activities and its relationship to secondary brain edema after brain injury. METHODS：NMDA receptor activities of neuronal membrane in the brain cortex were detected by using a radio ligand binding assay at different time after brain injury, the water contents of brain cortex were measured by dry-wet method.RESULTS：Bmax of NMDA receptor increased significantly and reached the peak at 30 minutes and the increase of water contents were obvious from 6 to 24 hours after brain injury; After treated with AP5(2-amino-5-phosphonlanoic acids), a selective NMDA receptor antagonist, the water contents and NMDA receptor antagonist, the water contents and NMDA receptor activities on neuron membrane decreased significanty compared to normal control group.CONCLUSION：Released glutamic acid might generate excitatoxic effect by activating NMDA receptor after brain injury.
MeSH Brain injuries; Brain edema; Rats
脑损伤后早期谷氨酸大量释放，作用于其受体而发挥兴奋性神经毒作用［1］，从而加重了脑损伤继发性损害，但主要通过何种受体发挥作用尚有争议。谷氨酸受体大致可分为N-甲基-D-天冬氨酸(N-methyl-D-aspartate, NMDA)受体和非NMDA受体两类，我们以NMDA受体为研究对象，对脑损伤及AP5治疗后不同时间伤侧大脑皮质NMDA受体活性与含水量进行测定，以探讨脑损伤后NMDA受体活性的时相变化与脑损伤继发性脑水肿的关系。
材料与方法
一、主要试剂：
［3 H］-L-谷氨酸(［3H］-L-Glu, 181.1×1010Bq/mmol, Sigma),3±2-羟基哌嗪丙基磷酸3-［(±)-2-ca-rboxypiperazin-4-yl］-propyl-[1] [2] 下一页
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