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活性维生素D对卵巢切除大鼠骨转换与骨质量的影响 |
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包方 李东 薛延 孙葆明 王芊 颜卉君
【摘要】 目的 研究1αOHD3对骨质疏松的预防治疗效果和作用机理。 方法 将40只雌性Wistar大鼠分为4组，以切除卵巢大鼠为模型，分别用0.04 μg/kg.d和0.5 μg/kg.d 1αOHD3灌胃给药12周。测定各组大鼠的血、尿生化指标，骨密度，生物力学和形态计量学等参数，以及骨粘连蛋白基因的表达水平。 结果 切除卵巢大鼠骨吸收和骨形成显著加强，骨密度明显降低，骨小梁结构恶化，骨生物力学性质显著降低。1αOHD3使大鼠骨量恢复，骨密度提高，骨小梁相对体积(TBV)增大，并使骨生物力学性质明显提高。0.5 μg/kg.d 1αOHD3对骨生物力学的效果优于0.04 μg/kg.d 1αOHD3的给药量。不同浓度1αOHD3对骨形成与骨吸收的作用不同：0.5 μg/kg.d 1αOHD3明显抑制骨转换；0.04 μg/kg.d 1αOHD3则促进骨转换。1αOHD3的浓度对骨的矿化速率无显著影响。大鼠去卵巢后，骨粘连蛋白(osteonectin, ON)mRNA表达水平降低，0.5 μg/kg.d 1αOHD3处理可恢复并促进ON mRNA表达。 结论 1αOHD3有明显增加骨密度和骨强度的作用。高浓度1αOHD3抑制骨转换，低浓度的1αOHD3促进骨转换和ON mRNA表达。
【关键词】 去卵巢大鼠 1αOHD3 骨转换 生物力学 骨形态计量学 骨粘连蛋白
EFFECTS OF 1αOHD3 ON THE BONE TURNOVER AND
QUALITY IN OVARIECTOMIZED RATS
Bao Fang, Li Dong, Xue Yan△, Sun Baoming, Wang Qian， Yan Huijun
(Laboratory of Biochemistry, Jishuitan Hospital, Beijing; Department of Biology,
Beijing Normal University, Beijing)
【Abstract】 Objective To investigate mechanisms of prevention and treatment of osteoporosis using 1αOHD3. Methods Forty female Wistar rats(3 months old) were divided into two groups, sham operated(sham) and ovariectomized. 1αOHD3 was given orally at the dose of 0.5 μg/kg.d and 0.04 μg/kg.d for 12 weeks, respectively. At the end of the experiments, serum levels of bone gel protein(BGP) and estrogen were determined by ELISA, serum alkaline phosphatase(ALP), calcium(Ca),phosphatase, tartrate-resistant acid phosphatase(TRAP) and urinary creatinine (Cr), hydroxyproline(HOP) and urinary Ca were determined by chemical method. Mechanical parameters for femur fracture in a three-point bending test, histomorphometric parameters and the expression of osteonectin(ON) mRNA were measured.Results Ovariectomy increased bone formation and resorption and decreased bone mineral density(BMD) of the femur and the 4t[1] [2] [3] 下一页
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