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大鼠实验性胃溃疡自愈期间胰岛A细胞的结构和功能变化 |
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黄 岩 梁文妹 石爱荣
【摘要】 目的 探讨胰岛A细胞参与溃疡自愈调节的可能途径。 方法 应用形态计量、放射免疫测定及透射电镜方法，研究大鼠实验性胃溃疡自愈期间胰岛A细胞的面数密度、超微结构及血清胰高血糖素的变化。 结果 与正常组及盐水组比较，A细胞面数密度于溃疡后6d、10d明显增加(P＜0.01)。溃疡后14d开始呈减小趋势，28d基本恢复正常。A细胞超微结构变化在溃疡后第6～14d较明显，主要表现为：(1)细胞核增大，异染色质减少，核周隙扩张；(2)部分A细胞粗面内质网增多，有的呈板层状排列，有的扩张成囊泡；(3)部分A细胞内分泌颗粒增多，大小不等，密度不一。血浆胰高血糖素水平从溃疡后14d到21d呈增高趋势(P＜0.01)，28d时接近正常。 结论 胰岛A细胞的形态和功能变化表明，它通过增加其细胞数目及增强其合成与分泌胰高血糖素的功能，积极参与了胃溃疡自愈的调节过程。
【关键词】 胰岛A细胞 形态计量学 超微结构 胰高血糖素 实验性胃溃疡 大鼠
THE CHANGES OF STRUCTURE AND FUNCTION OF
ISLET A CELLS DURING THE HEALING PROCESS
OF GASTRIC ULCER IN RATS
Huang YanΔ Liang Wenmei* Shi Airong**
(Department of Histology and Embryology,Weifang Medical College,Weifang ;
*Guiyang Medical College,Guiyang; **Beijing Medical University,Beijing 100083)
【Abstract】 Objective To explore the possible way by which the islet A cells take part in the regulation of the healing process of gastric ulcer in rats. Methods Using morphometry,electron microscopy and radioimmunoassay to study the changes the numerical density on area(NA) and ultrastructure of islet A cells and the level of glucagon during the healing process of gastric ulcer in rats. Results As compared with normal control group(NCG) and saline control group(SCG),the NA of A cells markedly increased(P＜0.01) during 6-10 days in experimental gastric ulcer group(EUG),then declined on the 14th day and recovered on the 28th day;the obvious ultrastructure changes occurred in A cells during 6-14 days after inducing gastric ulcer .The changes include that the nucleus was larger and rounder with the more euchromatin and dilated perinuclear space, the rough endoplasmic reticulum increased in the number or dilated into vacuole in some A cells; the number of secrete granules increased with different size and density.The level of plasma glucagon elevated remarkedbly(P＜0.01)during 14-21 days,and recovered on the 28th day. Conclusion The above mentioned f[1] [2] 下一页
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