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MRL lpr Thy1 1小鼠中一个新的T细胞亚群的发现 |
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宋永平 张永法 金雪兰 池原进
【摘要】 目的 研究MRL/lpr Thy1.1小鼠中T细胞的异常、异常T细胞的起源及其与淋巴腺病理的关系。方法 取出生后不同时期MRL/lpr Thy1.1小鼠及正常C57BL/6小鼠的造血淋巴组织，制备单细胞县液并用流式细胞技术检测其细胞表面标记，然后以t检验进行统计学处理。结果 发现MRL/lpr Thy1.1小鼠中存在着一个异常的、与淋巴腺病理密切相关的T细胞亚群，其表型为Thy1.1-TCRαβ+Ly5+,而且这一细胞亚群随着年龄增长而迅速增加，至19周龄，其可以占淋巴结细胞总数的80%。同时，淋巴结及脾脏中传统T细胞(Thy1.1+TCRαβ+Ly5-)随着Thy1.1-TCRαβ+Ly5+细胞的增加而逐渐减少。结果还表明，这一细胞亚群既不在胸腺也不在骨髓中分化成熟，但这一细胞亚群起源于某些异常骨髓造血细胞。结论 MRL/lpr Thy1.1小鼠中存在着一个起源于某些异常骨髓造血细胞的表型为Thy1.1-TCRαβ+Ly5+的随着年龄增长而迅速增加的T细胞亚群。
【主题词】 自身免疫疾病 T淋巴细胞亚群 骨髓移植 免疫母细胞淋巴结病
Identification of a novel T lymphocyte subpopulation in MRL/lpr Thy1.1 mice Song Yongping,Zhang Yongfa,Jin Xuelan,et al.First Affiliated Hospital,Henan Medical University,Zhengzhou 450052
【Abstract】 Objective To investigate T cells abnormality,the source of the abnormal T cells and the relationship between abnormal T cells and lymphadenopathy in MRL/lpr Thy1.1 mice.Methods The lymphohemopoietic cells were obtained from various week-old MRL/lpr Thy1.1 mice or normal mice.Then the surface markers of these cells were examined with FCM.Student t test was used to assess the statistical significance.Results In the experiments,an abnormal novel T lymphocyte subpopulation in MRL/lpr Thy1.1 mice was identified .The pattern of the subpopulation was of Thy1.1-TCRαβ+Ly5+.This subpopulation was associated with murine lymphadenopathy and increased sharply with ageing.The percentage of it in lymph node was about 80% in 19 week-old mice.In contrast,the conventional T lymphocytes(Thy1.1+TCRαβ+Ly5- T cells) in the lymph node and spleen decreased sharply while the abnormal subpopulation increased.By means of bone marrow transplantation,we found that this novel T cell subset was derived from some abnormal hemopoietic cells,although its differentiation sites remained obscure. Conclusions There exists Thy1.1-TCRαβ+Ly5+ T lymphocyte subpopulation in MRL/lpr Thy1.1[1] [2] [3] 下一页
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