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氯化镉对小鼠腹腔巨噬细胞功能的影响 |
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魏雪涛 薛彬 张铣
【摘要】 目的 观察镉对巨噬细胞功能的影响。方法 以0.3、0.6、1.2 mg/kg体重CdCl2连续给小鼠灌胃14天后进行巨噬细胞吞噬功能、产生NO的能力及产生H2O2等多个指标的检测。结果 在各剂量CdCl2作用下,小鼠腹腔灌洗液细胞数减少,与对照组比差异有显著性。巨噬细胞吞噬功能受到明显抑制,抑制率分别为21.8%、39.9%、44.4%,且呈现剂量-反应关系(r=-0.903 1)。巨噬细胞产生NO的能力也受到明显抑制,对照组为76.10±14.22 μmol/L,其于各组分别为61.90±27.83、36.67±23.86、46.60±13.33 μmol/L。巨噬细胞产生的O-2在三种剂量下都出现明显抑制,呈现剂量-反应关系(r=0.979 0)。巨噬细胞产生H2O2的能力仅在1.2 mg/kg剂量时出现明显抑制,与对照组比差异有显著性,但H2O2的产生与CdCl2剂量亦存在剂量-反应关系(r=-0.984 7)。结论 CdCl2在0.3、0.6、1.2 mg/kg剂量下对巨噬细胞吞噬功能,NO、O-2、H2O2等生物分子的产生均有明显抑制。因此认为CdCl2可直接影响巨噬细胞的抗原递呈及非特异性防御功能。
【关键词】 镉 巨噬细胞
Effects of Cadmium Chloride on the Function of Peritoneal Macrophage in Mice Wei Xuetao, Xue Bin, Zhang Xian. Department of Toxicology, Beijing Medical University, Beijing 100083
【Abstract】 Objective To study the effects of cadmium on macrophage function. Methods Many indicators, such as phagocytic function, abilities to produce nitrous oxide and hydrogen peroxide, in mice were determined 14 days after they were administered continuously with 0.3, 0.6 and 1.2 mg/kg cadmiuCell count decreased in the mouse peritoneal lavage with the effects of cadmium chloride, with a significant difference, as compared with the control group. Phagocytic function of macrophage was inhibited significantly, with inhibition rates of 21.8%, 39.9% and 44.4%, respectively for various doses of cadmium, in a dose-response pattern. The ability of microphage to produce nitrous oxide was significantly inhibited, also, with 61.90±27.83, 36.67±23.86, 46.60±13.33 μmol/L, respectively, as compared with 76.10±14.22 μmol/L in the control group. The ability of microphage to produce O-2 was obviously inhibited by three different doses of cadmium, showing a dose-response relationship (r=0.979 0). The ability of microphage to produce hydrogen peroxide was inhibited only at a dose of 1.2 mg/kg of cadmium, with a significant difference as compared [1] [2] [3] 下一页
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