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大鼠运动皮层甘氨酸免疫反应性神经元的分布及其超微结构特征 |
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高文军 郑则慧
摘 要：目的 探讨甘氨酸能神经元(Gly-ir)在大脑皮层内的分布及特征。 方法 用免疫组织化学与免疫电镜方法，在光镜与电镜水平观察甘氨酸免疫反应性神经元在大鼠运动皮层内的分布及其超微结构特征。 结果 甘氨酸免疫反应性神经元均为非锥体细胞，在运动皮层内Ⅰ～Ⅵ层均有分布，以Ⅰ～Ⅲ层较多，Ⅵ层最少，免疫反应性细胞以小细胞(41.5%)及中等大小细胞(44%)为主，也可见少量梭形细胞(13.5%)，而大细胞极少(1%)。这些不同形态与大小的细胞也有层次分布的特征：Ⅰ、Ⅵ层以小细胞为主，而Ⅱ～Ⅲ、Ⅴ层则以中等大小细胞为主。电镜下，甘氨酸免疫反应性神经元呈现中间神经元的特征：胞体小、核大浆少、核内陷。胞体上既可见具有扁平或多形囊泡的终末形成的对称型突触(79.7%)，也可见含球形、清亮囊泡的终末形成的非对称型突触(20.3%)。它的树突上也可见这两种突触类型，近端树突上与胞体相似，以对称型突触为主；而远端细小树突(直径＜1μm)上则以非对称型突触为主；呈现树突越细，非对称型突触越多，而对称型突触越少的特征。在运动皮层内未能找到Gly免疫反应性终末分布。 结论 首次观察到大脑运动皮层内确实存在甘氨酸能神经元，但目前尚不能肯定甘氨酸在皮层内是否具有神经递质的作用，有待进一步研究。
关键词：甘氨酸；免疫组织化学；电镜；运动皮层；大鼠
分类号：R322.81 文献标识码：A
文章编号：0529-1356(2000)01-1
DISTRIBUTION OF GLYCINE-IMMUNOREACTIVE PROFILES
IN THE RAT MOTOR CORTEX:A LIGHT AND
ELECTRON MICROSCOPIC STUDY
GAO Wen-Jun
(Shanghai Brain Research Institute,Chinese Academy of Sciences,Shanghai 200031,China)
ZHENG Ze-hui
(Shanghai Brain Research Institute,Chinese Academy of Sciences,Shanghai 200031,China)
Abstract：Objective It is known that glycine receptor(GlyR) exists extensively in motor cortex,but there is no report on the distribution and characteristics of glycinergic neurons in the cerebral cortex.Here it is the aim.Methods By means of immunocytochemical technique at the light and electronic microscopic level,we examined the morphological and ultrastructural characteristics of the glycinergic neurons in rat motor cortex.Results The Gly-ir neurons appeared in all layers,but mainly in layers Ⅰ-Ⅲ,fewer in layer Ⅴ and the least in layer Ⅵ.They were exclusively non-pyramidal shapes and were morphologically heterogeneous with soma size ranging from less than 10μm to greater than 15μm.However,the well-labeled neurons were predominantly small(41.5%) and medium(44%),some spindle-like(13.5%) and few large(1%) cells could also be observed.At the electron microscopic level,the somata of Gly-ir neurons received both of symmetric and asymmetr[1] [2] [3] 下一页
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