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Tenascin |
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杨大莉 黄文晋 梁喆 杨浩 鞠躬
摘 要：目的 研究FN6-8片段能否促进损伤神经元的突起生长。 方法 从包含有TN-C分子中FN6-8DNA序列的质粒中表达并纯化GST-FN6-8融合蛋白，以0.05mg/L的浓度加入培养的胚胎小鼠脊髓神经元的培养液中，对照组加入等量GST，然后液体石蜡封闭液面造成神经元缺气损伤，3d后做MTT实验，测神经元活性并图像分析神经元突起的长度。 结果 FN6-8组的神经元活性和神经元突起长度明显高于对照组。 结论 TN-C促进神经元活性及突起生长的功能可能由其FN6-8片段介导。
关键词：TN-C；FN6-8片段；缺气损伤；神经元活性；突起长度
分类号：Q513.2；Q954.52 文献标识码：A
文章编号：0529-1356(2000)01-5
THE EFFECT OF FN6-8 FRAGMENT PROTEIN OF TENASCIN-C
ON NEURONS FROM SPINAL CORDS
YANG Da-li
(Institute of Neuroscience,The Fourth Military Medical University,Xi an 710032,China)
HUANG Wen-jin
(Institute of Neuroscience,The Fourth Military Medical University,Xi an 710032,China)
LIANG Zhe
(Institute of Neuroscience,The Fourth Military Medical University,Xi an 710032,China)
YANG Hao
(Institute of Neuroscience,The Fourth Military Medical University,Xi an 710032,China)
JU Gong
(Institute of Neuroscience,The Fourth Military Medical University,Xi an 710032,China)
Abstract：Objective To study the function of FN6-8 of TN-C.Methods GST-linked FN6-8 fusion protein was expressed from the plasmid containing the DNA sequence of FN6-8 of TN-C.This fusion protein was added into culture medium of neurons from spinal cords of embryonic mice at the final concentration of 0.05mg/L.Equal volume of GST was added into the medium of the control group.Then liquid paraffin wax was added into the medium to create the air-deprived injury neurons.Three dyas later,MTT assay and image analysis were preformed to examine the activity and the neurite length of the neurons.Results FN6-8 can significantly promote the activity and neurite length of the gas-deprived neuron of the spinal cord.Conclusion The ability of FN-C to promote the viability and neurite length could be induced by FN6-8.
Key words：TN-C;FN6-8 fragment;neuronal activity;neurite length▲
Tenascin-C(TN-C)是发现较晚的细胞外基质大分子糖蛋白，功能复杂而强大，参与胚胎多种组织的发育[1] [2] 下一页
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