|
伽玛刀不同剂量照射正常大鼠一侧尾壳核后延髓内Fos蛋白的表达及变化 |
| |
饶志仁 吴声伶 邱建勇 徐欣 段晓勤 鞠躬
摘 要：目的 观察γ-刀不同剂量照射正常大鼠一侧尾壳核后3个月，远离靶区的延髓中尾段内Fos的表达和变化及与剂量的关系。 方法 分别用10Gy至100Gy 10个级别剂量照射大鼠一侧尾壳核，3个月后，用ABC法对延髓中尾段切片进行抗Fos蛋白的免疫组织化学反应。 结果 在延髓内脏带、三叉神经尾侧亚核、三叉神经间质核、延髓背侧网状核和下橄榄核等处出现多少不等的Fos阳性胞核，随着剂量的增加Fos阳性胞核的数量增加，范围扩大。在高剂量组的延髓腹外侧区出现3种Fos阳性细胞：胞核阳性、胞浆阴性；胞浆阳性、胞核阴性；胞核胞浆均为阳性。 结论 γ-刀照射前脑一个局部，在远离靶区的延髓中尾段出现Fos阳性反应，并与剂量成正相关。
关键词：γ-刀；延髓；Fos；延髓内脏带；免疫组织化学
分类号：R8 文献标识码：A
文章编号：0529-1356(2000)01-21
EXPRESSION AND CHANGE OF Fos IN THE MEDULLA
OBLONGATA OF RAT FOLLOWING EXPOSURE TO
VARIOUS DOSE OF GAMMA KNIFE
RAO Zhi-ren
(Institute of Neuroscience,The Fourth Military Medical University,Xi an 710032,China)
WU Sheng-ling
(Guang dong Minimally Invasive Neurosurgery Medical Center,Guang zhou 510630,China)
QIU Jian-yong
(Institute of Neuroscience,The Fourth Military Medical University,Xi an 710032,China)
XU Xin
(Guang dong Minimally Invasive Neurosurgery Medical Center,Guang zhou 510630,China)
DUAN Xiao-qin
(Institute of Neuroscience,The Fourth Military Medical University,Xi an 710032,China)
JU Gong
(Institute of Neuroscience,The Fourth Military Medical University,Xi an 710032,China)
Abstract：Objective To observe alterations in Fos protein expression in middle-caudal segment of the normal rat medulla oblongata after the unilateral caudate putamen nucleus was irradiated with different dosage of gamma knife.Methods The caudate putamen nucleus was irradiated with various dosage from 10Gy to 100Gy of gamma knife.The rats were sacrificed 3 months after irradiation and fixed and cut with cryostat.The sections of the medulla oblongata were reacted with anti-Fos protein immunohistochemical ABC method.Results At low dosage,Fos positive nucleus was observed in medullary visceral zone,caudal subnucleus of trigeminal nerve,intertrigeminal nucleus of trigeminal nerve,dorsal medullary reticular nucleus and i[1] [2] 下一页
上一个医学论文： 猴松果体分泌物释放入脑脊液主要途径的结构基础电镜研究
下一个医学论文： 成年大鼠纹状体区神经干细胞的分离培养
|
|
|
|
|
|
|
您现在的位置： 绿色健康网 >> 医学论文 >> 基础医学论文 >> 正文