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云南瑞丽静脉药瘾人群HIV1感染者毒株env基因V3区序列测定研究 |
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王 斌1 鲁晓晴1 路永波1 邵一鸣2 陈 筝2 赵全壁2 曾 毅2
摘要 为了解云南瑞丽静脉药瘾人群HIV1感染者毒株的分子流行病学特征，运用套式聚合酶链反应(nested PCR)对来自云南静脉药瘾人群的16株 HIV1 env基因V3区进行扩增，并对扩增片段进行DNA序列测定和分析。结果显示，与本地区共享序列相比，16株HIV1膜蛋白V3区氨基酸序列的株间变异为0%～23%，平均为7%。HIV1云南株膜蛋白V3区氨基酸共享序列与HIV1 SF2株及美欧株共享序列同源性在90%以上，而与海地、日本及非洲等地的代表毒株同源性较低。结果表明，在进化上这16株HIV1毒株间有非常密切的关系。这一地区的流行毒株在这一时期以美欧株、HIV1 SF2株及其衍生株为主。
关键词 人类免疫缺损病毒Ⅰ型 静脉药瘾者 分子流行病学
Sequence Analysis on HIV1 env V3 Rigion from HIV1 Infected Intravenous Drug Users in Ruili City,Yunnan Wang Bin*, Lu Xiaoqing,Lu Yongbo,et al.* Department of Molecular Virology, Medical College of Qingdao University,Qingdao 266021
Abstract To study the molecular-epidemiologic features of HIV1 epidemic strains from intravenous drug users (IVDUs) in Yunnan, nested primers polymerase chain reaction was developed for the amplification of env gene V3 fragment of HIV1 strains. DNA sequencing and analysis were performed with these fragments. Results showed that the variation of HIV1 gp120 V3 amino acids sequences among 16 HIV1 strains was averaged 7% (0%-23%),when comparing their consensus sequence. The amino acids consensus sequences of gp120 V3 region showed that more than 90% of homology compared with that of HIV1 SF2 strain and HIV1 American-European strain. However,lower homology with that of HIV1 strains from Haiti, Japan and Africa was revealed. The results suggested that there was a close relation among these 16 HIV1 epidemic strains isolated in Yunnan Ruili in terms of virus evolution. The epidemic HIV1 strains in the area were predominately American-European, SF2 and their derivatives in this period.
Key words Human immunodeficiency virus type 1 Injecting drug users Molecular epidemiology
HIV1膜蛋白V3区与病毒的免疫中和、免疫逃避、感染细胞间的融合及病毒的细胞嗜性［1，2］等有密切的关系，不同毒株间膜蛋白V3区氨基酸序列的变异可达50%以上［3］。文献表明，HIV1的膜蛋白V3区氨基酸序列的变异具有地理分布特征，并对毒株的分子流行病学追踪有重要的意义［4～6］。我们对1992～1994年来自我国云南瑞丽地区静脉药瘾人群(Injecting Drug Users,IDUs)中的16株HIV1毒株膜蛋白基因V3区进行了序列测定，确定了这些毒株膜蛋白V3[1] [2] [3] 下一页
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