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黄芪对感染病毒大鼠心肌细胞钙通道及钠钙交换载体的效应 |
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刘恭鑫 杨英珍 顾全保 郭棋 虞勇
摘 要 目的与方法：以柯萨奇B3病毒感染急性分离的 成年大 鼠心肌细胞，利用膜片钳记录技术了解黄芪对感染柯萨奇病毒后心肌细胞L型钙通道及钠钙 交换载体的影响，以探讨黄芪用于心肌炎治疗的作用机制。结果：黄芪不 影响正常心肌细胞L型钙通道电流的幅度，但可减缓L型钙通道电流的衰减。黄芪也不影响内 向NCE电流，但使外向NCE电流增加，从而使动作电位期间由NCE转运入细胞内的钙离子增加 。病毒感染增加心肌细胞L型钙通道电流的幅度，抑制内向钠钙交换电流，并使钠钙交换电 流的逆转电位负移。黄芪可抑制病毒感染细胞L型钙通道电流的增加， 减轻CVB3对内向NCE电流的抑制作用和 使负移的逆转电位正常化。结论：黄芪通过拮抗CVB3感染对L型钙通道 和钠钙交换电流的影响，可防止病毒感染可能导致的细胞内钙超载和异常电活动，从而对感 染细胞起到保护作用。
主题词 黄芪；柯萨奇病毒；钙通道；心肌；大鼠；
Effects of astragalus membranaceus on L type calcium channel
and Na+/Ca2+ exchange in adult rat cardiomyocytes
infected with coxsackie virus B3
LIU Gong-Xin,YANG Ying-Zhen,GU Quan-Bao,Guo Qi,Yu Yong
Key Laboratory of Viral Heart Diseases of Ministry of Public
Healt h,Zhongshan Hospital,Shanghai Medical University;
Shanghai Institute of Cardiovascular Diseases,Shanghai(200032)
Abstract AIM and METHODS: By patch clamp recording techniques to investigate the effects of astragalus membranaceus (AM) on L type voltage dep endent calcium channel (LVDCC) and Na+／Ca2＋ exchange (NCE) in a dult ra t cardiomyocytes infected with coxsackievirus B3(CVB3) so as to understand the mechanism of AM to cure myocarditis.RESULTS：AM did n ot affect the amplitude of calcium current,but decreased the “run down”of calc ium current;also,AM had no effect on inward NCE current,but increased outward NC E current.Both of the functions of AM to LVDCC and NCE would increase the calciu m inflow during the action potential. The LVDCC current in rat cardiomyocytes in fected with CVB3 increased;the inward NCE current decreased,and the reverse p otential of NCE was shifted to negative.AM could inhibit the increase of calcium current,mitigate the inhibition of CVB3 was shifted to negative.AM could inh ibit the increase of calcium current, mitigate the inhibition of CVB3 to inwa rd NCE current and normalize the negative shifted reverse potential.CONCL USIONS：By counteracting the eff[1] [2] [3] 下一页
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