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SOD对脑缺血 |
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任晓燕 葛宝林 曲忠森 张玉敏
摘 要 目的：探讨超氧化物歧化酶(SOD)对脑缺血再灌注 损 伤(I-R)大鼠皮层、海马、纹状体谷氨酸转运体功能的影响。方法：采用大 鼠3个血管夹闭、松夹复制脑I-R损伤模型。利用脑组织突触膜颗粒对［3H］-L-谷氨酸摄 入量 的测定及分光光度法观察SOD对皮层海马纹状体谷氨酸转运体功能、丙二醛(MDA)含量及SO D 活性的影响。结果：I-R组谷氨酸转运体的功能及SOD活性明显低于对照组( P＜0.05，0.01)，MDA含量明显高于对照组(P＜0.05，0.01)。SOD+I-R 组大鼠谷氨酸转运体的功能及SOD活性明显高于I-R组(P＜0.05， 0.01)；MDA含量明 显低于I-R组(P＜0.05， 0.01)。结论：SOD可改善I-R后脑组织谷 氨酸转运体的功能，其机制可能与清除自由基有关。
主题词 再灌注损伤；过氧化物脂质类；超氧化物歧化酶；大鼠
Influence of SOD on the function of glutamate transporter
in ra ts with injury of cerebral ischemia-reperfusion
REN Xiao-Yan, GE Bao-Lin, QU Zhong-Sen, ZHANG Yu-Min
Department of Pathophysiology, Medical College of Qingdao University, Qin gdao(266021)
Abstract AIM：To investigate the effect of superoxide dismutas e (SOD) on the function of glutamate transporter in rats with injury of cerebral i schemia-reperfusion.METHODS：A model of ischemia-reperfusion injur y(I-R) was performed by clipping three arteries and then by releasing them to rep erfuse blood into ischemic brain in rats. Glutamate transporter functions were s tudied by means of assay of ［3H］-L-glutamate uptake in synaptosomes, and malo ndialdehyde (MDA) contents and SOD activities in cerebral contex, hippocampus ,s triatum by spectrophotometry.RESULTS：Glutamate transporter functi on and SOD activity in I-R group significantly decreased (P＜0.05，P＜ 0.01) ;content of MDA significantly increased(P＜0.05， P＜0.01) compared with contr ol group. Glutamate transporter function and SOD activity in SOD group significa ntly increased (P＜0.05， P＜0.01);MDA content significantly decrease d(P＜0.05，P＜0.01) compared with I-R group.CONCLUSION：SOD can improv e glutamate transporter function of I-R rats. The mechanism might be related to eliminate free radicals.
MeSH Reperfusion injury; Lipid peroxides; Superoxide dismutas e; Rats
谷氨酸存在于兴奋性突触前神经末梢，遍布整个脑组织。其在突触间 隙中过多的积聚可产生神经毒性作用。正常情况下，神经元及神经胶质细胞膜上的谷氨酸转 运体在其毒性发生之前能很快清除突触释放的谷氨酸[1] [2] 下一页
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