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饲料锌过量对幼鼠脑发育及学习记忆的影响及其机理探讨 |
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孔祥英 刘力建 盛锡联 任榕娜 白家肆
【摘要】 目的 观察锌过量对幼鼠脑发育和学习记忆行为的影响。方法 从哺乳期开始建立过量锌幼鼠模型，采用电镜观察、神经生化和神经行为观察等方法。结果 随饲料锌含量的增加幼鼠大脑及海马重量减少，血清及海马锌增高。过量锌1、2组(ZE1、ZE2组)幼鼠主动回避反应(AAR)习得率及活动明显低于自由喂养组(AL组)及ZE2组的配对组(PF组)，ZE2组的恢复组(ZE2R组)AAR习得率也明显低于AL和PF组，但明显高于ZE2组。PF组与AL组AAR习得率差异无显著性，但活动较AL组多。ZE2组海马一氧化氮含量增加而生长抑素含量减少，脑组织兴奋性氨基酸和抑制性氨基酸总量增高，尤其是谷氨酸增高更为明显。ZE2组海马CA1区神经元、胶质细胞及细胞器均有不同程度变性、坏死，突触小泡破裂。结论 过量锌饲料明显影响幼鼠脑发育和功能，其机理是多方面的。
【关键词】 锌 脑 学习 记忆 行为
Effects of Excessive Zinc in Fodder on Brain Development and Abilities of Learning and Memory and Their Mechanisms in Young Rats Kong Xiangying, Liu Lijian, Sheng Xilian, et al. Department of Pediatrics, Southwest Hospital, The Third Military Medical University, Chongqing 400038
【Abstract】 Objective To study the effects of excessive zinc in fodder on brian development and abilities of learning and memory and to explore their mechanisms in young rats. Methods Animal models with excessive zinc were established in young Wistar rats during their lactation period. Rats were divided into five groups, Groups ZE1 and ZE2 were fed with excessive zinc, Group AL was freely fed with Flanagna fomula fodder added with zinc sulfate, Group PF was matched pair of Group ZE2 and Group ZE2R was fed with fodder same as Group AL after weaning. Ultrastructure was observed with electron microscopy, their free amino acids, nitrous oxide and somatostatin were measured with neurobiochemical methods, and their abilities to learn and memorize were examined. Results Weight of cerebrum and hippocampus in the rats reduced and zinc levels in their serum and hippocampus raised with the increase of zinc content in their fodder. Acute avoidance response (AAR) and movement in the young rats fed with excessive zinc in Groups ZE1 and ZE2 were obviously lower than in Groups AL and PF. AAR was significantly lower in Group ZE2R than that in Groups AL and PF, but significantly higher than that in Group ZE2. There was no signifcant diffe[1] [2] 下一页
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