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小鼠胚胎食管和肠上皮的体视学研究 |
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汪维伟 曾小川
摘 要：目的 研究消化管上皮发生的影响因素。 方法 用体视学法观测了11～17d ICR胎鼠连续切片中食管、十二指肠和结肠的管腔与上皮。部分切片用TUNEL法标记细胞凋亡作对照。 结果 (1)3段消化管的管腔面积与上皮面积比值均随胎龄增加而增高；上皮的凋亡小体密度(DAB)峰值均在分裂细胞密度(DMC)峰值之后；上皮细胞密度与DMC和DAB间未见一致的相关性；(2)食管的DAB峰值高于肠。食管上皮中凋亡小体分布于上皮各层，而肠上皮中主要在上皮游离面。 结论 (1)3段的管腔面积增长均快于上皮面积的增长。上皮大量增殖后凋亡，以适应其形态发生；(2)食管和肠扩大管腔的方式不同，而两者上皮中凋亡小体分布的不同可能与上皮隆起的形成有关；(3)胚胎消化管上皮细胞密度对上皮细胞分裂和凋亡的影响不明显。
关键词：食管；十二指肠；结肠；发育；体视学；胚胎小鼠
分类号：Q954.48 文献标识码：A
文章编号：0529-1356(2000)01-73
A STEREOLOGIC STUDY OF EPITHELIUM OF THE ESOPHAGUS
AND INTESTINES IN THE MOUSE EMBRYO
WANG Wei-wei
(Department of Histology and Embryology,Chongqing Medical University,Chongqing 400016,China)
ZENG Xiao-chuan
(Department of Internal Medicine of the 47th Hospital of PLA,Chengdu 610500,China)
Abstract：Objective To study the factors effected the development of epithelium in the digestive tract. Methods The cavity and epithelium of the esophagus、duodenum and colon in the serial sections of fetal ICR mice aged 11～17days were measured with the stereological method.The apoptosis was also showed by TUNEL method in some sections for contrast. Results (1)In the three segments of digestive tract,the value of cavity area/epithelial area all increased with the increase of the fetal age;the peak value of density of mitotic cells(DMC)all appeared before their peak value of density of apoptotic body(DAB);the unanimous correlation among the density of epithelial cells (DEC),DMC and DAB had not been found;(2)The peak value of DAB in the esophagus was higher than that of intestines.The apoptotic bodies distributed in all layers of the epithelial cells in the esophagus, but mainly located near the free surface of epithelium in the intestines. Conclusions (1)In the three segments of digestive tract,the increase of cavity area was more rapid than that of their epithelial area.The epithelial cells all proliferated in a large number first,and then appeared apop[1] [2] [3] 下一页
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