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人食管癌细胞核基质的研究 |
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陈海滨 邱殷庆 张锦堃 陈玲 任显辉 杨蕾
摘 要：目的 研究人食管癌细胞株EC1和EC18的核基质形态及蛋白成分。 方法 应用细胞的选择性抽提、DGD包埋-去包埋剂电镜制样观察核基质形态，SDS-聚丙烯酰胺凝胶电泳、双向电泳和免疫印迹等分析核基质蛋白成分。 结果 抽提后，两种细胞内均存在精细的核基质-核纤层-中间丝网架。低分化的EC1细胞核基质较高分化的EC18更细密。核基质蛋白电泳显示，两株细胞有数种相同的核基质蛋白成分，也有各自的特异性核基质蛋白。在免疫印迹分析中，使用抗人NuMA单克隆抗体检测到两株食管癌细胞中都存在NuMA蛋白。 结论 在食管癌细胞中，存在特异的核基质蛋白，核基质-核纤层-中间丝形成一个连续的体系，对维持细胞核的完整性和细胞功能起重要作用。
关键词：核基质；食管癌细胞；形态学；电泳
分类号：R735.1；Q279 文献标识码：A
文章编号：0529-1356(2000)01-65
THE NUCLEAR MATRIX IN HUMAN ESOPHAGEAL
CARCINOMA CELLS
CHEN Hai-bin
(Department of Histology and Embryology,Shantou University Medical College,Shantou 515031,China)
QIU Yin-qing
(Department of Anatomy,The Chinese University of Hong Kong,HongKong)
ZHANG Jin-kun
(Department of Histology and Embryology,Shantou University Medical College,Shantou 515031,China)
CHEN Ling
(Department of Histology and Embryology,Shantou University Medical College,Shantou 515031,China)
REN Xian-hui
(Department of Anatomy,The Chinese University of Hong Kong,HongKong)
YANG Lei
(Department of Anatomy,The Chinese University of Hong Kong,HongKong)
Abstract：Objective To investigate the nuclear matrix in two human esophageal carcinoma cell lines EC1 and EC18. Methods The fine structures of nuclear matrix were observed by DGD(diethylene glycol distearate) embeddment-free sections and the electrophoretic pattern of the nuclear matrix proteins was compared by SDS-PAGE,two-dimensional PAGE and Western blot analysis. Results The nuclear matrix-lamina-intermediate filament fine network remained after selective extraction.The nuclear matrix of the less differentiated EC1 cells was much thinner and denser than that of the more differentiated EC18.Both cell lines shared myriad common nuclear matrix proteins,but cell line specific nuclear matrix proteins was detected.Western blot analysis with anti-NuMA revealed that nuclear mitotic apparatus protein pre[1] [2] [3] 下一页
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