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斑蝥素及去甲斑蝥素诱导人红白血病K562细胞凋亡的细胞学研究 |
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孙震晓 魏育林 赵天德 李家实
摘 要：目的 研究抗癌药物斑蝥素及去甲斑蝥素诱导人红白血病K562细胞凋亡的细胞学变化特点。方法 观察药物作用后K562细胞形态的动态变化、一般及超微结构特征，并用流式细胞术分析斑蝥素及去甲斑蝥素诱导K562细胞凋亡与细胞周期的关系。结果 2mg/L斑蝥素及10mg/L去甲斑蝥素作用24h，部分K562细胞质膜突起，染色质异常凝集，原位复染显示这些细胞质膜的通透性没有增强，DNA琼脂糖凝胶电泳呈现细胞凋亡特有的“梯子”状带纹。电镜下，加药处理组中部分细胞染色质凝集成块，没有核膜包被，部分细胞染色质靠核膜边集呈新月形，胞浆浓缩，内质网结构正常或有扩张，呈现典型的凋亡细胞形态。流式细胞术分析结合细胞形态学研究表明，斑蝥素及去甲斑蝥素诱导K562细胞凋亡与细胞G2/M期阻滞相关。结论 斑蝥素及去甲斑蝥素能够诱导K562细胞凋亡，凋亡大部分发生在细胞周期的M期，也有少量发生在间期，是多点启动的。
关键词：斑蝥素；去甲斑蝥素；细胞凋亡；K562细胞；细胞周期
分类号：R979.1 文献标识码：A
文章编号：0529-1356(2000)01-56
STUDIES ON APOPTOTIC CYTOLOGY OF K562 HUMAN
MYELOID LEUKEMIA CELLS INDUCED BY
CANTHARIDIN AND NORCANTHARIDIN
SUN Zhen-xiao
(Liaocheng Teachers University,Shandong 252059,China)
WEI Yu-lin
(China-Japan Friendship Institute of Clinical Medical Sciences, Beijing 100029,China)
ZHAO Tian-de
(China-Japan Friendship Institute of Clinical Medical Sciences, Beijing 100029,China)
LI Jia-shi
(Beijing University of Traditional Chinese Medicine,Beijing 100029,China)
Abstract：Objective Studying the apoptotic cytology of K562 cells induced by cantharidin(CA) and norcantharidin(NCTD). Methods Observing morphological changes and ultrastructural features of treated K562 cells by phase contrast microscope and electron microscope.Relation between apoptosis and cell cycle of treated K562 cells was examined by fluorescence-activated cell sorting (FACS)analysis. Results Some K562 cells show cell membrane bubbling and nuclear chromatin compacting after treated with 2mg/L CA or 10mg/L NCTD for 24h.The membrane permeability of treated K562 cells proved no increase by counterstaining in situ.An apoptosis characteristic DNA“Ladder” detected by using agarose gel electrophoresis.Several masses of condensed chromatin without nuclear membrane were observed in some treated K562 cells,while chromatin of some other cells tended to marginate in crescents aroun[1] [2] 下一页
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