|
三磷酸腺苷诱导人白血病细胞U937凋亡的电镜观察 |
| |
吕桂芝 胡颖 张志谦 林仲翔
摘 要：目的 为探讨三磷酸腺苷(ATP)对人白血病细胞U937的促凋亡作用。 方法 应用ATP(0.23g/L)作用于人白血病细胞U937(human histocytic lymphoma),(1)用白细胞计数器每日计数细胞和计算抑制率；(2)用流式细胞分析仪检测ATP对U937细胞周期改变和凋亡的影响；(3)苏木精-伊红染色和电子显微镜检测凋亡小体的形成过程；(4)用苯胺黑染色检测凋亡小体的生命本性。 结果 (1)ATP对U937细胞的增殖有明显的阻抑作用，加药48h后增殖的抑制率可达43%以上；(2)ATP处理的U937细胞周期发生改变，G1期细胞数明显增多，ATP作用24h时G1期前出现亚二倍体峰——凋亡峰，凋亡细胞数为3.4%，作用48h时凋亡细胞数增多为22.7%；(3)ATP处理的U937细胞首先在核内染色质浓缩成半月形贴近核膜，逐渐向核膜外移动，进入胞浆内再移向质膜内外面，紧贴质膜外面再逐步脱离细胞体，进入细胞基质中，成为游离的凋亡小体。凋亡小体有双层核膜及内质网和线粒体与深染的染色质；(4)凋亡小体拒染黑色素。 结论 (1)ATP对U937细胞的增殖有明显的阻抑作用；(2)ATP诱导U937细胞凋亡；(3)ATP阻断U937细胞于G1期，G1期前出现凋亡峰并出现凋亡小体(4)凋亡小体是有生命的结构；(5)ATP是U937细胞凋亡的有效促进剂。
关键词：ATP；人白血病细胞；凋亡
分类号：R733.7 文献标识码：A
文章编号：0529-1356(2000)01-48
ELECTRON MICROSCOPIC OBSERVATION ON ATP
INDUCED U937 CELLS APOPTOSIS
LÜ Gui-zhi
(The School of Oncology,Beijing Institute for Cancer Research,Beijing Medical University,
Beijing 100034，China)
HU Ying
(The School of Oncology,Beijing Institute for Cancer Research,Beijing Medical University,
Beijing 100034，China)
ZHANG Zhi-qian
(The School of Oncology,Beijing Institute for Cancer Research,Beijing Medical University,
Beijing 100034，China)
LIN Zhong-xiang
(The School of Oncology,Beijing Institute for Cancer Research,Beijing Medical University,
Beijing 100034，China)
Abstract：Objective In order to study the effects of ATP on U937 cells (human histocytic lymphema). Methods Cell culture,flow cytometry,HE staining,Nigrosine staining and electron microscopy were used. Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment; (2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma[1] [2] 下一页
上一个医学论文： 肿瘤组织中端粒酶活性的研究
下一个医学论文： 脂多糖诱发急性肺炎时中性粒细胞碱性磷酸酶细胞化学定位观察
|
|
|
|
|
|
|
您现在的位置： 绿色健康网 >> 医学论文 >> 基础医学论文 >> 正文