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辅佐细胞在磷酸一乙脂激活 T细胞中的作用 |
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黄岚 葛锡锐
摘 要：目的：研究辅佐细胞在非肽类抗原MEP激活γδT细胞中的作用及机制。方法：用花环沉降法分离出正常人外周血中的T细胞，通过细胞计数和FACS，观察MEP激活γδT细胞时辅佐细胞的作用。结果：MEP激活γδT细胞需要有IL-2的持续存在。MEP和IL-2联合作用于T细胞可少量激活其中的γδT细胞，但与IL-2的单独作用无明显差异。加入辅佐细胞能大大促进MEP和IL-2联合激活γδT细胞的作用，且明显强于IL-2的单独作用。辅佐细胞分泌的上清对MEP激活γδT细胞无明显促进作用。结论：MEP激活γδT细胞需要辅佐细胞的参与，可能主要通过与T细胞间的直接接触而起作用。
关键词：γδT细胞 磷酸一乙脂 辅佐细胞
分类号：R371.4
Role of accessory cells in the activation of γδT cells induced by monoethyl phosphate
HUANG Lan
Shanghai Institute of Cell Biology,Chinese Academy of Sciences,Shanghai 200031
GE Xi-Rui
Shanghai Institute of Cell Biology,Chinese Academy of Sciences,Shanghai 200031
Abstract：Objective：Study of the role of accessory cells in the activation of γδT cells by nonpeptidic antigen monoethyl phosphate(MEP).Methods： T cells from peripheral blood of healthy donors have been isolated by means of E-rosette,then the cellular requirement for the proliferation of γδT cells activated by MEP has been investigated by flow cytometry.Results： The continuous presence of IL-2 was required for the proliferation of γδT cells in response to MEP.The combination of IL-2 and MEP stimulated a few of γδT cells among the whole T cells,but the expansion had no significant difference compared with IL-2 alone.However,the addition of accessory cells obviously enhanced the proliferation of γδT cells induced by MEP and IL-2,and the effect was obviously greater than that by IL-2 alone.The supernatant of accessory cells had no obvious effect on the expansion of γδT cells stimulated with MEP.Conclusion： Accessory cells are necesssary in the activation of γδT cells induced by MEP.Moreover,it is possible that accessory cells provide the necessary costimulatory factors for γδT cell proliferation b[1] [2] [3] 下一页
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