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中国部分地区新型肝炎病毒TTV感染的分子流行病学 |
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周育森 董京芳* 周乙华 黄呈辉* 何忠平* 王海涛
【摘要】 目的 了解我国部分地区新型肝炎病毒TTV感染的分子流行病学、TTV在人群中的感染分布状况,探讨TTV在肝炎发病中的作用和地位。方法 采用PCR方法检测血清标本中TTV DNA，克隆测定不同地理株TTV的部分基因序列，分析其基因变异情况。结果 检测了南方（广东深圳和江苏南京）和北方（北京和辽宁沈阳）的112例非甲～非庚型肝炎病人，其中TTV DNA阳性者48例，阳性率42.9%，而102例甲～庚型肝炎病人中阳性率为2.9%（χ2=42.8,P<0.01）。ALT异常而无甲～庚型肝炎病毒感染标志的献血员中，TTV DNA阳性率（34.6%）明显高于ALT正常献血员的阳性率（16.8%，χ2=4.5, P<0.01）。 南方株（广东深圳TTVCHN002、南京TTVNAN001 ）和北方株（北京TTVSHB015）病毒间同源性在98%以上，日本发表的序列同源性也高于97%。结论 我国北方和南方地区均存在TTV感染。TTV感染与ALT异常有极为密切的关系，可能是导致非甲～非庚型肝炎的重要病原。正常人群中存在TTV感染者，类似于HBsAg的所谓“慢性携带状态”。
【关键词】 肝炎病毒 聚合酶链式反应 TT病毒
Molecular Epidemiology of TT Virus Infection in Some Parts of China Zhou Yusen, Dong Jingfang, Zhou Yihua, et al. Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beinjing 100071
【Abstract】 Objective To study the molecular epidemiology of new hepatitis TT virus (TTV) infection, its distribution in population and its role in pathogenesis of hepatitis in some parts of our country. Methods TTV DNA in serum samples was detected by nested-polymerase chain reaction, partial gene of various geographic strains of TTV was cloned and sequenced, and their genetic variation was analyzed. Results Forty-four of 112 cases with non-A to G hepatitis from Shenzhen, Guangdong province, Nanjing, Jiangsu province, Beijing and Shengyang, Liaoning province were positive for TTV DNA with a positive rate of 42.9%, but only three of 102 cases of hepatitis A～G was positive, with a positive rate of 2.9% (χ2=42.8, P<0.01). Positive rate of TTV DNA was significantly higher in blood donors with abnormal ALT but without infection markers of hepatitis A～G (34.6%) than in those with normal ALT (16.8%) (χ2=4.5, P<0.05). Sequencing analysis showed that more than 98% of their nucleotides were analogous between strains of TTVCHN002 from Shenzhen and Nanjing, and TTVSHB015 from Beijing, and more than 97% analogous between the above-men[1] [2] [3] 下一页
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