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膈下迷走神经切断术抑制腹腔给予LPS诱导的延髓内脏带内Fos表达 |
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杨志军 饶志仁 邱建勇 鞠躬
【摘要】 目的 研究迷走神经在免疫系统至延髓内脏带(MVZ)通路中的可能性。方法 对雄性SD大鼠行膈下双侧迷走神经干切断术或假切断手术，存活4周后再经腹膜腔注入免疫刺激剂脂多糖(LPS)或无菌生理盐水(NS)，3h后处死，用免疫组织化学ABC法对MVZ进行Fos染色。结果 假手术组大鼠腹腔给予LPS后，MVZ可发现大量Fos表达；膈下迷走神经切断术组大鼠腹腔给予LPS后，MVZ内的Fos表达量则明显减少。结论 迷走神经参与外周免疫信息向中枢的传递，外周免疫信息可经迷走神经传至MVZ，MVZ是“免疫-脑通讯”的中继站，机体内存在“迷走神经→MVZ”神经免疫调节通路。
【关键词】 延髓内脏带；神经免疫调节；膈下迷走神经切断术；Fos；脂多糖；免疫组织化学
【中图分类号】R322.81 【文献标识码】A
【文章编号】0529-1356(2000)02-97
SUBDIAPHRAGAMATIC VAGOTOMY INHIBITS Fos EXPRESSION IN
THE MEDULLARY VISCERAL ZONE AFTER INTRAPERITONEAL
ADIMINISTRATION OF LIPOPOLYSACCHARIDE
YANG Zhi-jun,RAO Zhi-ren,QIU Jian-yong,JU Gong
(Institute of Neuroscience,the Fourth Military Medical University, Xi an 710032,China)
【Abstract】 Objective To test the possibility that the vagus nerve is involved in the communication between the immune system and the medullary visceral zone(MVZ). Methods Male Sprague-Dawley rats were received either subdiaphragmatic vagotomy or sham operation.Four weeks later,rats were challenged with pyrogen-free saline or lippolysaccharide(LPS) by intraperitoneal route,and sacrificed 3hrs later.Medulla sections were processed for Fos immunoreactivity using ABC method. Results Intraperitoneal LPS caused robust Fos expression within the MVZ in the sham surgery groups and this response in the MVZ was inhibted in the vagotomized rats. Conclusions This study suggested that the abdominal vagus nerve consituted an important immune signaling pathway from immune system to the MVZ,and MVZ was a relay station in the immune-to-brain communication.MVZ might play a prominent role in the neuroimmunomodulation via vagus-to-MVZ neuroimmunomodulatory pathway.
【Key words】 Medullary visceral zone;Neuroimmunomodulation;Subdiaphragamatic vagotomy;Fos;Lipopolysaccharide;Immunohistochemistry
现已公认中枢神经系统与免疫系统之间存在相互调节，但中枢神经系统哪些部位参与免疫调节是一个尚未解决的问题。有文献证实，下丘脑室旁核(PVN)的催产素(oxytoci[1] [2] 下一页
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