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大鼠三叉神经脊束核尾侧亚核 |
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陶发胜 高蓉 李云庆
【摘要】 目的 观察臂旁核(PBN)内向丘脑腹后内侧核(VPM)投射神经元和三叉神经脊束核尾侧亚核(Vc)向臂旁核投射纤维和终末的分布,以及两者之间的突触联系。 方法 HRP逆行追踪与生物素葡聚糖胺(BDA)顺行追踪相结合的双标技术,标记结果分别在光镜或电镜下观察。 结果 将HRP注入VPM后,在光镜下可见HRP逆标神经元主要位于同侧的臂旁外侧核、Klliker-Fuse(KF)核和臂旁内侧核。将BDA注入Vc后,BDA顺标纤维和终末也主要见于同侧臂旁外侧核、KF核和臂旁内侧核,对侧丘脑VPM、丘脑后核和丘脑胶状质核也有BDA顺标纤维和终末,但臂旁核内的BDA顺标纤维和终末明显多于丘脑诸核团。在臂旁核内,HRP逆标神经元的主要分布区与BDA顺标纤维和终末的主要分布区重叠。电镜下在臂旁核内可见,Vc向臂旁核投射的BDA顺标纤维和终末与臂旁核向丘脑VPM投射的HRP逆标神经元形成以非对称性为主的轴-树和轴-体突触。 结论 大鼠中枢内存在Vc-PBN-VPM间接联系通路,该通路在面口部躯体感觉信息(包括伤害性信息)的传递和调控过程中可能发挥重要作用。 【关键词】 臂旁核;丘脑腹后内侧核;三叉神经脊束核尾侧亚核;突触联系;伤害性刺激;大鼠 【中图分类号】 R322.81 【文献标识码】 A 【文章编号】 0529-1356(2000)02-108
STUDIES ON THE CAUDAL SPINAL TRIGEMINAL NUCLEUS- PARABRACHIAL NUCLEI-THALAMUS INDIRECT CONNECTION PATHWAY IN THE RAT
TAO Fa-sheng,GAO Rong,LI Yun-qing (Department of Anatomy and K.K.Leung Brain Research Centre,the Fourth Military Medical University,Xi an 710032,China)
【Abstract】 Objective To investigate:(1)distribution of neurons projecting from the parabrachial nuclei(PBN) to the thalamic ventral posteromedial nucleus(VPM) and the fibers and terminals originating from trigeminoparabrachial projection neurons;(2)synaptic connection between trigeminoparabrachial terminals and parabrachiothalamic projecting neurons. Methods Horseradish peroxidase(HRP) retrograde tracing combined with biotinylated dextran amine (BDA) anterograde tracing technique was used.The labeling results were observed under light of electron microscope,respectively. Results After injection HRP into the unilateral VPM,HRP retrogradely labeled nueronal cell bodies were mainly found in the ipsilateral lateral parabrachial nucleus(PBL),Klliker-Fuse(KF) nucleus and medial parabrachial nucleus(PBM).After injection BDA into the unilateral Vc,BDA anterogradely labeled fibers and terminals were mainly encountered in the ipsilateral PBL,KF nucleus,PBM,and contralateral VPM,thalamic posterior nucleus and thalamic su[1] [2] [3] 下一页 上一个医学论文: 胶质细胞源性神经营养因子在成年大鼠背根节分布的免疫组织化学研究 下一个医学论文: 反义GFAP逆转录病毒表达载体的构建及其对反应性星形胶质细胞的作用
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