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二甲基甘氨酸对脊髓神经元的作用 |
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ervical part of the spinal cord was dissected and a length of 5 mm from the middle of the cervical enlargement of the spinal cord was selected for the study. The tissue was processed and paraffin sections of 3 micron thickness were stained with cresyl violet. The slides were coded to avoid bias.
From each animal 8-10 sections at intervals of 15-20 microns (total of 100 sections) were selected for analysis of the gray matter, white matter and total area measurement. The sections were viewed on a screen using a video camera attached to Biolux binocular microscope. The total area ventral to a line drawn across the central canal of the spinal cord was measured using a grid attached to the screen(Fig. 1a). Similarly the total area of the gray matter was measured in the same region(Fig.1a). The area of white matter was calculated by subtracting the area of gray matter from the total area.
Similarly, the neuron counts were done from the ventral halves of the spinal cord(right and left sides)using the same equipment. The interval between the two selected sections was 15-18 microns. The number of neurons in the range of 15-25 microns(maximum diameter) and above 25 microns were counted. Neurons below 15 microns and without a clear cell boundary and nucleus were excluded. The diameter was measured using a graduated transparent scale(Fig.1b). All the neurons without differentiating them into different classes of motor neurons and inter neurons were counted. The data was analyzed using the one-way ANOVA test.
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