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可溶性HLA |
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兰炯采① 石宁 郑世荣 陈强 刘红雨 武大林 曹琼 杨崇礼
摘 要 目的：sHLA-Ⅰ可预测器官移植排斥反应及监控某些疾病。为了介绍sHLA-Ⅰ类抗原定量检测技术及中国人sHLA-Ⅰ正常值。方法：采用ELISA双抗夹心法，以W6/32 mAb包被酶标板，加被检血清，再加β2m-HRP及底物显色。以不同浓度sHLA-Ⅰ标准品的结果绘出标准曲线并测定113例正常人sHLA-Ⅰ含量。结果：ELISA法可以准确定量测定血清中sHLA-Ⅰ，中国人sHLA-Ⅰ正常值为789.27±506.32 ng/ml。结论：ELISA法检测sHLA-Ⅰ具有特异、敏感及重复性。
关键词 可溶性人类白细胞抗原(sHLA) 酶联免疫吸附试验(ELISA) 定量检测
中国图书分类号 R392-33 R392.4
Quantification of serum-soluble HLA class Ⅰ antigens
LAN Jiong-Cai, SHI Ning, ZHENG Shi-Rong et al.
Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College, Chengdu 610081
Abstract Objective:sHLA-Ⅰ antigens may predict the onset of rejection after organ transplantation and monitor some diseases. The method of quantification of sHLA-Ⅰ antigens and its normal level in Chinese were reported. Methods:Sandwich ELISA assay was used. After microtiter plate coated by W6/32 mAb, serum was added, followed by incubation with anti-β2m-HRP and the color development. Standard curve was obtained by quantification of sHLA-Ⅰstandard reagent in series dilution. The amount of sHLA-Ⅰantigens was examined in 113 normal healthy volunteers. Results: sHLA-Ⅰ antigens can be exactly quantificated by sandwich ELISA assay. The level of sHLA-Ⅰ antigens in normal individual is 789.27±506.32 ng/ml. Conclusion: Sandwich ELISA assay is special, sensitive and reproducible for sHLA-Ⅰ antigens, and the results can be compared each other only when reagents and protocols are the same.
Key words Soluble human leukocyte antigens(sHLA) Enzyme linked immunosorbent assay(ELISA) Quantification
1970年van Rood在正常人血清中检测到可溶性人类白细胞-Ⅰ类抗原(sHLA-Ⅰ)。sHLA-Ⅰ见于血清、体液、乳汁、汗液和尿液中，由α链和12 kD的β2微球蛋白(β2m)以共价键结合形成异源二聚体。α链有44、39、36、34 kD四型［1］。由于sHLA-Ⅰ在器官移植排斥反应及某些疾病的转归中具有监控作用，故1992年(巴黎)及1993年(美国凤凰城)召开了国际研讨会［1，2］。至今国内少见sHLA-Ⅰ的定量检测及中国人正常值的文献，现将我们的研究报道如下。
1 材料与方法
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