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组织蛋白酶D在乳腺良 恶性病变中的表达及意义 |
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崔猛胜 刘秉义 季德刚 李晨玉①
摘 要 目的：探讨组织蛋白酶D在乳腺良、恶性病变中的表达及意义。方法：用ABC法研究93例乳腺癌、31例乳腺良性病变及6例正常乳腺组织Cath-D的表达。结果：乳腺癌中43例(46.24%)细胞浆染色阳性，而全部良性病变及正常乳腺组织均阴性。Cath-D表达阳性的乳腺癌其肿瘤体积及淋巴结转移率均高于Cath-D表达阴性者。结论：Cath-D可以作为反映乳腺癌细胞增殖状态的细胞生物学因子，其过度表达提示乳腺癌恶性程度高。
关键词 乳腺癌 免疫组化 组织蛋白酶D
中国图书分类号 R392.66
The expression and significance of cathepsin D in begin and malign breast disease
CUI Meng-Sheng， LIU Bing-Yi, JI De-Gang et al.
The Third Clinical Hospital of Norman Bethune University of Medical Science， Changchun 130031
Abstract Objective:Discussed the expression and significance of cathepsin-D in breast begin and breast carcinomas. Methods:Examined the expression of cathepsin-D in 93 cases of breast carcinomas, 31 cases of breast begin disease and 6 cases of normal breast tissue with the ABC immunohistochemical technique. Results:Demonstrate that the cytosol staining is positive in 43 cases (46.24%) of breast cancer but negative in all of the begin breast disease and te normal breast tissue. The size of tumor and the ratio of lymphnode metastasis in the breast cancer in which the expression of cathepsin-D were positive are higher than that the expression were negative. Conclusion: Discussion cathepsin-D can be used as a biological factor to reflect the proliferation status of carcinoma cells. The carcinoma cells in which cathepsin-D expression is positive have higher malignant degree.
Key words Breast cancer,prognosis Immunohistochemical Cathepsin D
本研究应用ABC法研究了93例乳腺癌，31例乳腺良性病变及6例正常乳腺组织中Cath-D的表达情况并探讨其在乳腺癌预后的意义。
1 材料与方法
1.1 材料 93例乳腺癌组织全部取自白求恩医大三院1994～1995年手术切除并经病理证实(其中浸润性导管癌49例、单纯癌25例、髓样癌13例、粘液癌3例、导管内癌3例)，另取31例良性病变组织(纤维腺瘤12例、囊性增生病7例、乳头状瘤9例、乳腺炎3例)及6例正常乳腺组织作为对照。乳腺癌年龄29～63岁，平均年龄48.5岁。
1.2 试剂 兔抗人Cath-D为IYMED公司产品；猪抗兔IgG与ABC试剂均为美国DAKO公司产品。
1.3 方法 所有组织均用10%福尔马林液固定，石蜡包埋，4 μm连续切片3张。切片常规二甲苯脱腊、梯度乙醇水化后用三羟甲基氨基甲烷缓冲液(TBS)清洗3次，使用标准猪血清(1∶20)封闭，37℃孵育30 min，TBS清洗3次，[1] [2] [3] 下一页
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