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异丙肾上腺素致大鼠心肌坏死时心肌细胞核钙转运功能的变化 |
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王培勇 姚兴海 苏静怡 汤健 唐朝枢
摘 要 目的：观察ISO心肌坏死时大鼠心肌细胞核钙转运系统的改变。方法：大鼠心肌坏死模型采用皮下注射ISO制备，心肌细胞核采用差速离心和密度梯度离心分离纯化，用酶学方法鉴定核的纯度及测定ATPase活性，用45Ca2+同位素法测定核钙摄取。结果：与对照组相比，ISO坏死心脏组织呈显著的损伤和坏死，心系数增加35%(P＜0.01)，心肌组织MDA和钙含量分别增加57%和80%(P＜0.01)。与对照组相比，ISO心肌坏死细胞核Ca-ATPase的［Ca2+］反应Vmax降低27.5%(P＜0.01)，Ka无显著变化；对［ATP］反应的Vmax无显著变化，而Km增加61.7%(P＜0.05)；ISO组45Ca2+摄取显著降低(P＜0.01)，Vmax与对照组相比降低46.1%(P＜0.01)，Km无显著变化。结论：坏死心肌细胞核钙转运系统功能显著降低，可能与心肌坏死时细胞核功能紊乱有关。
主题词 心肌；坏死；钙；细胞核；Ca2+转运ATP酶
Dysfunction of myocardial nuclear calcium transport in isoproterenol induced myocardial necrosis in rats
WANG Pei-Yong， YAO Xing-Hai,SU Jing-Yi,TANG Jian, TANG Chao-shu
Laboratory of Pathophysiology,Cardiovascular Basic Research Institute,Beijing Medical University, Beijing(100083)
Abstract AIM:Myocardial nuclear calcium transport was investigated on the model of isoproterenol(ISO)induced cardiac hypertrophy.METHODS:Rats were given injections of ISO subcutaneously(69 μmol.kg-1.d-1. for 3 days).Myocardial nuclei were purified with sucrose density centrifugation and identified zymologically,the activity of Ca2+-ATPase was measured zymologically and calcium uptake was assayed with ［45 Ca2+］isotope.RESULTS:ISO-injured heart presented obvious myocardial injury and necrosis. The ratio of heart weight to body weight in ISO group increased by 35%(P＜0.01).MDA and calcium content in myocardial tissue increased by 57% and 80% respectively(P＜0.01 compared with control).Vmax to ［Ca2+］and Km to ［ATP］of ATPase activity decreased by about 27.5%和61.7% respectively(P＜0.05).Significant decrease of nuclear［45Ca2+］uptake was observed and Vmax value was lowered by 46.1% compared with that of control(P＜0.01),but Km value remained unchanged.CONCLUSION：Dysfunction of nuclear calcium transport in isoproterenol induced myocardial injury.
MeSH Myocardium; Necrosis; Calcium;Cell nucleus;Ca2+-transporting ATPase
Ca2+是细胞内重要的第二信使，不仅调节细胞的功能代谢，而且在细胞核内还对细胞分化[1] [2] [3] 下一页
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