|
单抗5C5 |
| |
马凤蓉 朱立平 张立新 李波 张淑珍 王汛 李国燕 赵方萄 崔莲仙
作者单位：100005 北京，中国医学科学院基础医学研究所，
中国协和医科大学基础医学院免疫学系
本项目受国家自然科学基金重点项目资助，项目号为39630300
【 摘要 】目的 鉴于5C5 cDNA(613bp)的2～450bp 与 HSMRP17 mRNA(1008bp)的
560～1008bp的同源性高达95%，且后者编码的蛋白质(MRPL12)也在细胞活化后表达，
本研究旨在探究两者是否属同一物。 方法 多个人B细胞株用单抗5C5-G1作间接免
疫荧光染色，激光扫描共聚焦显微镜观察。 结果 5C5-G1单抗识别分子表达于
膜下近膜胞浆处、也可能在膜表面，但非单纯在膜上，而MEPL12只表达于线粒体。另外，
前B细胞Nalm-6与单抗5C5-G1反应阴性，活化前期B细胞Daudi和3D5的反应强阳性，分
化晚期B细胞SKW6为弱阳性。 结论 5C5 cDNA与HSMRP17 mRNA 不属同一基因；单抗5C5-
G1识别的分化抗原的表达在B细胞分化过程中有变化。
【 主题词 】 抗体，单克隆 5C5 cDNA B细胞胞膜 显微镜检查
The differentiation antigen recognized by McAb 5C5-G1 is expressed on t
he surface of activated B cells Ma Fengrong,Zhu Liping,Zhang Lixin, e
t al.Department of Immunology,Institute of Basic Medical Sciences,C
hinese Academy of Medical Sciences and Peking Union Medical College,B
eijing 100005
【 Abstract 】 Objective Since the homology between 2-450bp of 5C5 c
DNA (GenBank accession number U25041)and 560-1008bp of HSMRP17 mRNA (Ge
nBank accession number X79685)reaches 95% and protein MRPL12 encoded
by HSMRP17 mRNA is expressed and accumulated during the G1 phase of grow
th-stimulated cells,the objective of the experiment is to estimate if t
he proteins encoded by the two genes are the same. Methods The indirect i
mmunofluorescence staining was done on several human B cell lines with
McAb 5C5-G1 and examination was carried out under a laser scanning confo
cal microscope. Results It was observed that the protein recognized by
McAb 5C5-G1 was only found on the membrane of 3D5 cells,and not in the cyto
plasm, while MRPL12 was only found in mitochondria. Besides,the react
ion between human pro-B cell line Nalm-6 and McAb 5C5-G1 was negative,the
reaction between Daudi, 3D5 cells and the McAb was strongly positive,a
nd SKW-6 cell was week[1] [2] 下一页
上一个医学论文： 人重组C3片段通过IL
下一个医学论文： 细胞性一氧化氮供体的建立及其对肿瘤细胞凋亡的诱导效应
|
|
|
|
|
|
|
您现在的位置： 绿色健康网 >> 医学论文 >> 基础医学论文 >> 正文