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上皮生长因子受体与非小细胞肺癌肿瘤免疫及转移的相关关系 |
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钟雪云 肖柳珍 廖剑辉
摘 要 目的与方法： 采用链霉菌抗生物素蛋白——过氧化物酶连结法(LSAB)免疫组化和图象定量分析技术检测50例非小细胞型肺癌 (其中伴有淋巴结转移者32例， 不伴转移者18例)上皮生长因子受体(EGFR)的表达。 结果：EGFR表达与肿瘤间质淋巴细胞浸润(TIL)呈负相关(P＜0.01)，EGFR高表达时，TIL浸润少，肺癌的转移率高(83%)。EGFR的表达与肺癌的组织类型明显相关(P＜0.05)，但与肺癌的分化程度与转移无相关。结论：肿瘤细胞内EGFR的表达程度与体内肿瘤免疫机能密切相关，二者共同影响肺癌细胞的生长、分化和转移。
主题词 癌， 非小细胞肺； 上皮；生长物质；淋巴细胞，肿瘤浸润
Correlations of EGFR to neoplasm immunity and
non-small cell lung cancer metastases
ZHONG Xue-Yun , XIAO Liu-Zhen, LIAO Jian-Hui
Department of Pathology, Medical College,Jinan University ,Guangzhou (510632)
Abstract AIM and METHODS: The expression of epidermal growth factor receptor (EGFR) in 50 cases of non-small cells lung cancer cells (32 had node metastases and others without ) was examined by means of labeled streptoaviodin-brotin complex (LSAB) immunohistochemical technic, image analysis. RESULTS: Correlations were found between EGFR expression and histological types (P＜0.05), but the expression of EGFR in lung cancer had no correlation with its differeniation, and had significant negative correlation with tumour infiltrating lymphocytes (TIL) (P＜0.01), while EGFR expression was high the node metastatic percentage was 83% TIL was much less. CONCLUSION: The expression of EGFR was closely correlated with immuno-system in vivo, they both had effects on growth, differentiation , and metastasis of cancer cells.
MeSH Carcionma, non-small cell lung; Epithelium; Growth substances; Lymphocytes, tumor-infiltrating
上皮生长因子受体(epidermal growth factor receptor, EGFR)是由原癌基因c-erb B-1编码的跨膜糖蛋白的受体。EGFR胞内片段具有蛋白酪氨酸激酶活性，能使细胞内微环境发生变化，导致核内原癌基因c-fos、c-myc表达来刺激细胞生长、增殖［1, 2］。肿瘤转移与EGFR表达相关［3～5］。为了探讨EGFR表达与肿瘤免疫以及肿瘤转移之间的关系,用链霉菌抗生物素蛋白——过氧化物酶连结法(labeled streptoaviodin-brotin comlex, LSAB)免疫组化和图象定量分析技术，对伴有淋巴结转移和不伴淋巴结转移的50例非小细胞肺癌进行EGFR的表达检测，同时检测这些非小细胞肺癌标本的肿瘤浸润淋巴细胞(tumour infiltrating lymphocytes, TIL)，探讨EGFR与人类肺癌转移的关系及其相关机制。
材料与方法
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