|
检测HBVDNA Ig |
| |
周裕林 彭宣宪 柳珑 肖军生 岑岭 杨天赐
摘 要：目的：建立一种免疫捕捉法PCR技术，研究血清免疫复合物中不同Ig类型抗体结合HBV的情况，并初步探讨其意义。方法：以羊抗人μ、γ、α链的IgG为捕捉抗体， 再利用PCR扩增捕捉物中的HBVDNA。结果：成功地建立了检测 HBV-DNA/IgM、IgG和IgA-TCIC的免疫捕捉法PCR技术。该技术特异性强、敏感性高、重复性好，可以显著提高HBVDNA的检出率。同时发现，结合HBV的抗体的Ig类型组合有明显差异，三者均阳性最高。结论：说明乙肝患者血清免疫复合物中有较多的病毒颗粒。研究HBVDNA/Ig-TCIC对乙肝发病机理的深入阐明可能具有重要意义。
关键词：免疫捕捉法PCR 循环免疫复合物 HBVDNA
分类号：R392-33
Immuno-capture PCR for HBVDNA/Ig-two-component-determined circulating immune complexes
ZHOU Yu-Lin
（Department of Biology,the Education Minister Key Laboratory for Cell Biology and Tumor Cell Engineering, Xiamen 361005）
PENG Xuan-Xian
（Department of Biology,the Education Minister Key Laboratory for Cell Biology and Tumor Cell Engineering, Xiamen 361005）
LIU Long
（Department of Biology,the Education Minister Key Laboratory for Cell Biology and Tumor Cell Engineering, Xiamen 361005）
Abstract：Objective: To establish an immuno-capture PCR for the study of classes of immunoglobulins complexed with HBV and of their significance.Methods:Using goat-IgG to human μ、γ、α chain as capture antibodies and prime-specific PCR as a detecting method.Results:An immuno-capture PCR had been established to detect HBVDNA/Ig-TCIC (HBVDNA/IgM-TCTC,HBVDNA/IgG-TCIC, HBVDNA/IgA-TCIC). This method was specific,sensitive and reproducible,by which more patients with positive HBVDNA could be found. It was also characted that there were significantly different positive rates among the types of combinations of IgM,IgG and IgA, in which the group with the three of them was hightest.Conclusion:These findings suggested that more HBV may be hide within circulating immune complexes and the study of HBV-DNA/Ig-TCIC may play an important role in more understanding of pathogenesis in patients with hepatitis B.
Key words：Immuno-capture PCR Circulating immune complex HBVDNA▲
按双特异性免疫复合物(TCIC)的新概念，病原体/Ig-TCIC与抗原/Ig-T[1] [2] 下一页
上一个医学论文： 抗人M和N型GPA特异性单克隆抗体的制备和鉴定
下一个医学论文： 自学
|
|
|
|
|
|
|
您现在的位置： 绿色健康网 >> 医学论文 >> 基础医学论文 >> 正文