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大鼠消化道促性腺激素释放激素受体mRNA的原位杂交研究 |
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姚兵 黄威权 蒲若蕾 孙岚 张荣庆 王雷
【摘要】 目的 探讨大鼠消化道是否存在GnRH受体mRNA及其定位。 方法 用原位杂交组织化学法。 结果 胃底腺壁细胞、胃小凹上皮细胞可检测到较强的GnRH受体mRNA杂交信号。3段小肠绒毛上皮细胞、小肠腺细胞可检测到较强的GnRH受体mRNA杂交信号。盲肠、结肠和直肠的粘膜上皮细胞、肠腺上皮细胞也能检测到GnRH受体mRNA杂交信号。信号物质均分布在胞质内，胞核呈阴性。 结论 大鼠消化道能合成GnRH受体。GnRH也是一种胃肠激素，它由消化系统自身合成，又作用于胃肠道参与胃肠功能的调节。
【关键词】 GnRH受体mRNA；原位杂交；消化道；大鼠
【中图分类号】 R322.4 【文献标识码】 A 【文章编号】 0529-1356(2000)03-250
In situ HYBRIDIZATION STUDY OF GONADOTROPIN
RELEASING HORMONE RECEPTOR mRNA IN RAT
DIGESTIVE TRACT
YAO Bing HUANG Wei-quan PU Ruo-lei SUN Lan ZHANG Rong-qing WANG Lei
Department of Histology and Embryology, the Fourth Military Medical University, Xi an 710032,China
Department of Biology, Tsinghua University, Beijing 100084,China
【Abstract】 Objective To study whether gonadotropin releasing hormone receptor(GnRHR) mRNA exists in rat digestive tract and it s cellular localization. Methods In situ hybridization using digoxigenin-labeled oligonucleotide probes was performed. Results Parietal cells of gastric gland and epithelium of gastric pit were detected to have GnRHR mRNA hybridization signal. In small intestine, the villou s epithelial cells and glandular cells could be detected to have strong GnRHR mRNA hybridization signal. In caecum、colon、rectum,very strong GnRHR mRNA hybridization signal was also detected in mucosa epithelium and glandular epithelium.The signals distributed in cytoplasm of all positive cells with negative nuclei. Conclusion The GnRHR mRNA was widely distributed in rat digestive tract with distributive pattern similar to those of GnRHR in pituitary. It suggests that the GnRH is produced by digestive system and response for modulating the digestive function as a gastrointestinal hormone.
【Key words】 GnRHR mRNA;In situ hybridization;Digestive tract;Rat
GnRH及其受体可在下丘脑-垂体轴外表达已被公认［1］，我们先前的研究已证明，胃肠胰系统能自身合成GnRH［2］，而且证实胃肠胰系统及颌下腺均含有GnRH受体免疫反应性物质［3，4］。本实验采用原位杂交法，观察大鼠消化道能否表达GnRH受体mR[1] [2] [3] 下一页
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