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肝纤维化启动期大鼠血清对肝星形细胞TGF |
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蒙一纯 丁霞 贲长恩 郭顺根 王泰玲
【摘要】 目的 研究大鼠血清与肝星形细胞表达TGF-β1的关系，为临床的早期诊断和中医药治疗肝纤维化提供新的思路。 方法 制备正常大鼠血清、免疫性肝纤维化大鼠血清和益气活血复方药物血清，培养肝星形细胞(HSC)，激光共聚焦显微镜定量分析其TGF-β1的表达。 结果 (1)造模3周时大鼠血清使培养的HSC中TGF-β1表达显著增强，正常鼠血清、造模5周和7周(即纤维化形成后)血清没有此作用；(2)添加益气活血药物血清可使造模3周鼠血清刺激HSC高水平表达的TGF-β1作用消失。 结论 (1)造模3周鼠血清中含有强烈的刺激HSC激活的物质；(2)活血化淤方剂的作用靶点是血液中的活性物质，通过拮抗血液中HSC活化物质达到抗纤维化的作用。
【关键词】 肝星形细胞；转化生长因子-β1；肝纤维化；血清
【中图分类号】 R575.2 【文献标识码】 A 【文章编号】 0529-1356(2000)03-257
INITIAL FIBROTIC SERUM AFFECT ON TGF-β1 EXPRESSION
IN HSC DURING LIVER FIBROGENESIS OF RAT
MENG Yi-chun DING Xia BEN Chang-en WANG Tai-ling
Department of Histology and Embyology, Beijing University of Traditional Chinese Medicine,Beijing 100029,China
Chian-Japan Friendship Hospital,Beijing 100029,China
【Abstract】 Objective To study initial fibrotic serum effect on TGF-β1 expression in HSC and the mechanism of anti-liver fibrosis by the chinese herb studied. Methods Hepatic stellate cells were cultured respectively in DMEM media with normal rat sera, hepatic fibrogenic rat sera induced by porcine serum for 3 weeks, 5 weeks and 7 weeks, and the sera containing Yiqi Huoxue agents.TGF-β1 expression was quantified by laser common focus microscope. Results TGF-β1 expression was prodominantly increased by cultured HSC in the DMEM media plus the 3 weeks model rat sera, TGF-β1 expression was scarce in the DMEM media plus the model rat sera for 5 and 7 weeks. TGF-β1 expression decreased obviously to zero in the DMEM media together with 3 weeks model rat sera and pharmacological sera. Conclussion There is a strong stimulating substance in the 3 weeks rat serum for TGF-β1 expression in HSC.The prescription of Yiqi Huoxue may have an effect on anti-liver fibrosis through inhibiting the stimulating substance of TGF-β1 expression in the serum.
【Key words】 HSC;TGF-β1；Liver fibrosis;Serum
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