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小鼠卵巢 型胶原 间质金属蛋白酶和层粘连蛋白的合成 分布 |
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钱晓菁 许增禄徐健 徐园园
【摘要】 目的 研究Ⅳ型胶原、层粘连蛋白(laminin, LN)和间质金属蛋白酶-2(matrix metalloproteinase-2,MMP-2)在不同年龄小鼠卵巢中的分布和变化模式。 方法 利用生物素-抗生物素蛋白间接免疫荧光方法，从蛋白水平观察Ⅳ型胶原和LN的分布和变化；利用地高辛标记的Ⅳ型胶原和MMP-2基因探针进行原位杂交，从mRNA水平观察Ⅳ型胶原和MMP-2的分布和变化。 结果 出生5d小鼠卵巢的卵泡基膜和卵巢表面上皮(ovarian surface epithelium, OSE)基膜处已有少量Ⅳ型胶原和LN分布，基质细胞和OSE中也有分布。Ⅳ型胶原和MMP-2的mRNA在基质细胞和OSE有表达。发育期小鼠卵巢中卵泡基膜和OSE基膜中Ⅳ型胶原和LN的含量随年龄的增加而呈增加趋势，分布也趋于连续。颗粒细胞、膜细胞、OSE中都有Ⅳ型胶原和LN分布，也有Ⅳ型胶原和MMP-2 mRNA的表达，其表达水平明显比5d时高。成熟期小鼠卵巢Ⅳ型胶原和LN的含量较高。12个月的小鼠卵巢已开始退化，基膜细胞外间质(extracellular matrix,ECM)的数量减少，MMP-2 mRNA的表达量无明显变化。 结论 Ⅳ型胶原和LN在小鼠卵巢发育过程中含量和分布发生着动态变化，可能对卵泡发育起重要作用。
【关键词】 卵巢Ⅳ型胶原；层粘连蛋白；间质金属蛋白酶-2
【中图分类号】 R322.6+5，R329.2+4 【文献标识码】 A 【文章编号】 0529-1356(2000)03-231
SYNTHESIS AND DISTRIBUTION OF TYPE Ⅳ COLLAGEN, MATRIX
METALLOPROTEINASE AND LAMININ IN MOUSE OVARIES
QIAN Xiao-jing, XU Zeng-lu, XU Jian, XU Yuan-yuan
(Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China)
【Abstract】 Objective To investigate the distribution of type Ⅳ collagen and LN and the expression of type Ⅳ collagen and MMP-2 genes. Methods Biotin-avidin DCS system indirect immunofluorescence technique, in situ hybridization with diglabeling type Ⅳ collagen and MMP-2 cDNA probe were used. Results In the ovary of 5-day-old neonatal mouse, a small amount of type Ⅳ collagen and LN fluorescence was seen in the basement membrane of follicle and OSE. The expression of type Ⅳ collagen and MMP-2 was observed inside OSE and stromal cells. In 15day to 2 month old mouse ovaries, the contents of ECM components increased with advancing age, and the distribution of basement membrane componets were smoothly than before. The mRNA expressions of type Ⅳ collagen and MMP-2 was markedly increased during this peroid. OSE, granulosa and thecal cells were the cells, which contributed to the synthesis of those ECM components and MMP-2. In 2 to 6 month [1] [2] 下一页
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