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Losartan对神经肽Y诱导血管平滑肌细胞增殖的逆转作用 |
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黄少华 刘建康 陈敏生
［摘 要］ 目的：观察神经肽Y（NPY）与血管紧张素受体拮抗剂losartan对体外培养的血管平滑肌细胞（VSMC）中PCNA、PDGF、c-myc癌基因表达的影响，从分子生物学的角度探讨NPY在高血压、动脉粥样硬化发生中的作用和losartan的逆转作用。方法：应用荧光免疫组化定量技术(ACAS570共聚焦激光扫描显微细胞仪)。结果：NPY刺激VSMC增殖，使其PCNA、PDGF和c-myc癌基因的表达明显高于对照组；losartan则可逆转NPY对VSMC增殖的刺激作用，使PCNA、PDGF和c-myc癌基因表达的平均荧光值降低。结论：NPY促进VSMC的增殖，其作用与高血压的发生有关；而losartan对高血压的治疗作用可能与拮抗NPY对VSMC增殖的刺激作用有关。
［主题词］ 神经肽Y； 血管紧张素Ⅱ； 受体， 血管紧张素； 肌， 平滑， 血管
［中图分类号］ Q 253 ［文献标识码］ A
［文章编号］ 1000-4718(2000)03-0211-03
The stimulating effects of neuropeptide Y on cultured arterial smooth muscle cell proliferation and losartan treatment
HUANG Shao-hua, LIU Jian-kang， CHEN Min-sheng
(Department of Cardiology, the First Affiliated Hospital, Guangzhou Medical College, Guangzhou 510182， China)
［Abstract］ AIM: To observe expression of proliferating cell nuclear antigen (PCNA), platelet derived growth factor (PDGF) and c-myc in cultured vascular smooth muscle cells which is effected by neuropeptide Y (NPY) and losartan (the receptor blocking agent of angiotensin Ⅱ) therefore exploring effects of NPY on the generation of hypertension and its relationship with losartan reverse treatment in molecular biology. METHODS: Applied the method of quantitative immunocytochemistry through ACAS570. RESULTS: 24 hours exposure of vascular smooth muscle cell to NPY caused an increase in expression of PCNA, PDGF and c-myc respectively. But losartan could reverse these expressions by NPY, decreased the expression of PCNA, PDGF and c-myc. CONCLUSION: NPY is closely related to the generation of hypertension. But losartan could reverse these effects of NPY.
［MeSH］ Neuropeptide Y; Angiotensin Ⅱ; Receptors, angiotensin; Muscle, smooth, vascular
研究资料表明：losartan对高血压心肌肥厚、高血压血管重塑具有良性的治疗效应［1］。有人推测这一作用除了losartan可拮抗血管紧张素Ⅱ受体的作用外，还很可能涉及losartan和神经体液因素中神经肽Y(neuropeptide Y，NPY)间相互作用及对促进VSMC增殖癌基因的调控。本研究利用体外培养的血管平滑肌细胞(vascular smooth muscle cell，VSMC)模型，观察NPY[1] [2] 下一页
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