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诱导型一氧化氮合酶与Dahl |
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taining the following antibiotics: Mezlin, 30 mg/d, (Miles, Westhaven CT) and penicillin G, 5 000 U/d. Rats were placed in a temperature controlled room with a 12 h light/dark cycle. Both catheters were exteriorized at the dorsal nape of the neck, and were connected to a dual channel infusion swivel (Instech Laboratories Inc., Plymouth Meeting, PA). Saline solutions were infused with a syringe pump (AJ5803) through a 0.22 μm filter (Cathivex, Millipore Corporation, Bedford, MA). The arterial catheter was filled with 1 000 U/mL heparin and connected to a Cobe pressure transducer (Lakewood CO) and in turn to a pressure amplifier. Pulsatile arterial pressure signals from the amplifier were sent to a digital computer through an analog-to-digital converter and were sampled 500 Hz for 4 seconds of each minute throughout the entire 24h period. Arterial pressure were determined from these data samples.
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