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抗氧化剂对黄曲霉毒素染毒的转基因小鼠肝肿瘤的防护 |
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王金莹 王立新 林三仁
【摘要】 目的 研究抗氧化剂2,3-叔丁基-4-羟基茴香醚（BHA）对黄曲霉毒素（AFB1）染毒的乙型肝炎病毒（HBV）转基因小鼠肝肿瘤发生的防护作用。方法 对AFB1染毒和未染毒的49只转基因小鼠与48只非转基因小鼠，测定肝醌还原酶(QR)和谷胱甘肽S转移酶(GST)活性、肝丙二醛(MDA)含量、肝氧自由基浓度(OFR)。结果 染毒的转基因小鼠BHA组肝腺瘤发生率17%，癌变率0，显著低于普通饲料组的肝腺瘤发生率（67％）和癌变率（22％）。与普通饲料组比较，BHA使肝OFR和MDA含量显著降低；BHA组肝QR和GST活性平均升高3～7倍。结论 BHA能显著诱导肝脏生物转化Ⅱ相酶活性，清除氧自由基，抑制变异肝细胞生长，可能与抑制或延缓小鼠肝癌的发生、发展进程有关。
【关键词】 小鼠，转基因 丁羟茴香醚 黄曲霉毒素B1 肝肿瘤，实验性
Protection against Synergistic Hepatocarcinogenesis of Hepatitis B Virus Expression and Aflatoxin B1 by Antioxidant 2(3)-tert-4-hydroxyanisole (BHA) in HBV Large Envelope Transgenic Mice WANG Jin-ying, WANG Lixin, LIN Sanren.Department of Gastroenterology, The Third School of Clinical Medicine, Beijing Medical University, Beijing 100083
【Abstract】 Objective To investigate the effects of 2(3)-tert-4-hydroxyanisole (BHA) on protection against synergistic hepatocarcinogenesis of aflatoxin B1 (AFB1) and hepatitis B virus (HBV) expression in HBV large envelope transgenic mice. Methods Protective effects of dietary antioxidant BHA on life span was observed in 49 cases of HBV transgenic mice and 48 cases of non-transgenic mice by determinations of enzyme activities and liver MDA, and by direct detection of liver oxidative free radicals (OFR) using electron spin resonance (ESR). Results In the HBV transgenic mice which exposed to AFB1, the incidence of hepatocellular adenoma was 17% (2/12), but no carcinoma was found in BHA group. In the regular diet group, that was greater with 67% (6/9) of adenoma and 22% (2/9) of carcinoma. BHA could decrease significantly the concentrations of liver MDA and OFR, compared with those with regular diet. The activities of quinone reductase and glutathione S-transferase in liver cytosols increased by 3-7 times, as in the controls, in response to BHA. Conclusions Addition of BHA to the diet resulted in significantly elevation of phase II enzyme activities in liver. BHA could directly eliminate liver OF[1] [2] 下一页
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