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酒精代谢酶基因型在日本双生子中的分布 |
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屈卫东 山县然太朗 吴德生 张本忠 张英宁 浅香昭雄 饭岛纯夫
【摘要】 目的 为预防酒精相关性疾病发生，调查了酒精代谢酶控制基因在日本双生子中的分布。方法 以饱和酚法提取DNA，应用限制性片段长度多态性分析技术检测了92个日本双生子的酒精脱氢酶2（ADH2）和乙醛脱氢酶2（ALDH2）基因型，根据基因型差异筛选敏感个体。结果 ADH2和ALDH2基因分布符合Hardy-weinberg等式。ADH2基因的3种基因型分别是ADH21/ADH21(1.1％)、ADH21/ADH22(44.6％)和ADH22/ADH22(54.3％)。ALDH2的基因型分别为ALDH21/ALDH21(41.3％)、ALDH21/ALDH22(39.1％)和ALDH22/ALDH22(19.6%)。ADH2和ALDH2基因频率分别为0.255、0.745和0.609、0391。结论 异常纯合的ADH2基因和纯合的ALDH2基因占优势。个体携有ADH21/ADH22和ALDH21/ALDH21、ADH22/ADH22和ALDH21/ALDH21者可视为敏感个体。
【关键词】 基因型 双生 醇脱氢酶 醛脱氢酶
Distribution of Genotypes of Alcohol Dehydrogenase 2 and Aldehyde Dehydrogenase 2 in Japanese Twin Children QU Weidong*, Zentor Yamagata, WU Desheng, et al. *Department of Environmental Health, School of Public Health, Shanghai Medical University, Shanghai 200032
【Abstract】 Objective In order to prevent alcohol related deseases, this study investigated the distribution of the genes controlling alcohol metabolism in Japan′s twin. Methods Restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) technique was used to measure the control gene of alcohol metabolized enzymes and the genotypes of alcohol dehydrogenase 2 (ADH2) and aldehyde dehydrogenase 2 (ALDH2), which were distributed in Japan′s twins. At the same time, according to the difference in genotypes, the sensitive individuals were screened from the study subjects. Results The distribution of ADH2 and ALDH2 genes were conistent with the Hardy-weinberg equation. The three genotypes of ADH2 gene were ADH21/ADH21(1.1%), ADH21/ADH22(44.6%) and ADH22/ADH22(54.3%). And those of ALDH2 gene were ALDH21/ALDH21(41.3%), ALDH21/ALDH22(39.1%) and ALDH22/ALDH22 (19.6%). The frequency of ADH2 and ALDH2 genes was 0.255, 0.745 and 0.609, 0.391 respectively. Conclusion Not only the distribution of genotypes of ADH2 and ALDH2 is known, but also the sensitive individuals are found, which can help prevent alcohol related disease.
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