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甲基叔丁基醚对大鼠肝组织基因表达的影响 |
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周伟 叶舜华 朱运松
【摘要】 目的 甲基叔丁基醚（MTBE）是一种新型的汽油添加剂，动物试验显示其具有一定的致癌性。为了解其动物致癌性的可能机制，我们检测了经MTBE亚慢性染毒的大鼠肝组织中原癌基因c-myc基因和功能基因谷胱甘肽S-转移酶P（GST-P）基因的表达情况。方法 40只雄性SD大鼠，体重180～200 g，随机分为4组。将MTBE溶于适量豆油中，灌胃染毒，染毒剂量分别为200 mg/kg，600 mg/kg，1 000 mg/kg和对照组。每天1次，每周5天，共13周。动物肝组织于液氮速冻后，一步法提取总RNA。随机引物法地高辛标记c-myc和GST-P探针，与RNA进行点杂交，图像分析仪分析结果。结果 大鼠肝组织中c-myc基因表达水平明显增高，GST-P基因表达未见增强。结论 MTBE可明显诱导c-myc基因的高表达，提示其具有促进细胞增殖的作用，这是其动物致癌性的可能机制之一。
【关键词】 醚类 基因,MYC 谷胱甘肽转移酶 杂交
Effects of Methyl Tertiary Butyl Ether on Liver Tissue Gene Expression in Rats ZHOU Wei*, YE Shunhua, ZHU Yunsong. *Department of Environmental Health, School of Public Health, Shanghai Medical University, Shanghai 200032
【Abstract】 Objective Methyl tertiary butyl ether (MTBE) is a new gasoline additive, with certain carcinogenecity in animal experiments. To study its possible mechanism of carcinogenesis in animals,expression of protooncogene c-myc and functional gene glutathione S-transferase-P (GST-P) in the liver tissues of rats chronically exposed to MTBE was deteited. Methods Forty male SD rats with body weights of 180-200 grams were randomly divided into four groups and gavaged with MTBE dissolved in soybean oil with doses of 1 000, 600, 200 and 0 mg/kg, respectively, once daily and five days a week for 13 weeks. Liver tissues of the animals were frozen quickly in liquid nitrogen and their total RNA extracted with one-step method. The probes of c-myc and GST-P were labeled with digoxin by random primer method, and dots hybridization with RNA was used. Results Levels of expression of c-myc gene in the liver tissues of rats increased significantly, but not for those of GST-P. Conclusion MTBE can induce the higher expression of c-myc gene, which suggests it can promote cell proliferation-one of possible mechanisms of carcinogenesis in animals.
【Key words】 Ethers Genes, myc Glutathione transferase Hybridization
甲基叔丁基醚(MTBE)是一种新型的汽油添加剂，被用来提高汽油燃烧效率，增加辛烷值[1] [2] 下一页
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