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慢性重型乙型肝炎患者血清病毒全长基因特点及其临床意义 |
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nd cloned from four treatment naive CHB patients and four treatment naive CSHB patients.Fourteen to sixteen clones per sample were selected,sequenced and analyzed by bioinformatics software.The measurement data was compared by independent-samples t test and count data was analyzed by x2 test. Results Totally 120 HBV fulllength genome sequences were obtained.All the patients had either genotype B or C virus monoinfection.One hundred percent clones(60/60)from CSHB patients showed mutations including G1896A,A1762T/G1764A(one patient even carried A1762T/G1764A/C1766T mutations),T1753C/G and start codon mutations in preS2,preS1,which were more common than those from CHB patients(46/60,76.7%;x2=15.85,P<0.01).The quasispecies complexity and diversity were higher in CSHB patients than CHB patients within full-length genome,S,X,P genes and reverse transcriptase region,but lower within C gene at both nucleotide and amino acid levels.But the difference were not statistically significant in all regions.Conclusion The mutation frequency and quasispeeies heterogeneity in HBV genome are higher in CSHB patients than in CHB patients,which may play a role in the severe exacerbation of CHB and needs further investigation in large scale studies.
Keywords：Hepatitis B virus;Hepatitis B,chronic;Mutation;Genetic heterogeneity;Severe hepatitis B;Quasispecies
基金项目：国家"十一五"科技重大专项(2008ZX10002-007、2009ZX10004-314)
作者单位：黄素园（200025,上海交通大学医学院附属瑞金医院感染科）
于德敏（200025,上海交通大学医学院附属瑞金医院感染科）
刘峰（200025,上海交通大学医学院附属瑞金医院感染科）
陈立（200025,上海交通大学医学院附属瑞金医院感染科）
韩悦（200025,上海交通大学医学院附属瑞金医院感染科）
李新华（200025,上海交通大学医学院附属瑞金医院感染科）
张欣欣（200025,上海交通大学医学院附属瑞金医院感染科）
参考文献：
[1]尹桂兰.病毒性肝炎防治新进展.北京:中医古籍出版社,2005:192-193.
[2]徐道振.病毒性肝炎临床实践.北京:人民卫生出版社,2006:243.
[3]中华医学会传染病与寄生虫病学分会、肝病学分会.病毒性肝炎防治方案.中华传染病杂志,2001,19:56-62.
[4]Gunther S,Li BC,Miska S,et al.Hepatitis B virus genomic hete上一页 [1] [2] [3] 下一页
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