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CD150在成人乙型肝炎疫苗无应答者体外外周血单个核细胞中的表达 |
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BsAg,anti-HBs,HBeAg,anti-HBe and anti-HBc).After recruitment,all 202 subjects received another standard scheme(0,1 and 6 month)revaccination.The blood samples were collected 7 months later after the first injection of revaccination to detect anti-HBs titer.The PBMCs were isolated from 18 adult non-responders(anti-HBs titer<10 mIU/mL)and 18 adult responders(antiHBs titer≥100 mIU/mL).CD150 expression on cell surface was analyzed by flow cytometry.SAS package was used for t test and spearman rank correlation analysis.Results After rHBsAg stimulation,the percentage of PBMCs expressed CD150 was significantly higher in non-responders (39.20%±10.66%)than responders(23.73%±12.41%)(t=2.1947,P<0.05).The same trend was also observed in rHBsAg stimulated C133+CD4+T cells,but the difference was not statistically significant(49.64%±11.94%vs 37.73%±11.02%)(t=1. 7175,P>0.05).After phytohaemagglutinin (PHA)stimulation,the percentage of CD150-positive PBMCs was also significantly higher in non-responders (39.21%±7.37%)than responders(23.18%±12.68%)(t=2.2835,P<0.05).CD150 expressions in both PBMCs and CD3+CD4+T cells were negatively correlated with anti-HBs titer after rHBsAg stimulation (r=-0.726,P<0.05).Conclusion Activation of CD150 may contribute to the non-response to hepatitis B vaccine.
Keywords：Hepatitis B vaccines;Signaling lymphocytic activation molecule(CD150);Peripheral blood mononuclear cells
基金项目：广东省自然科学基金资助项目(10151008901000011);广东省科技计划资助项目(2008B030301048)
作者单位：林潮双（510630,广州,中山大学附属第三医院感染病科）
顾琳（510630,广州,中山大学附属第三医院感染病科）
刘静（510630,广州,中山大学附属第三医院感染病科）
王向阳（广东出入境检验检疫局附属广东国际旅行卫生保健中心）
麦剑威（广州南沙横沥医院内科）
林润南（广州南沙横沥医院内科）
高志良（510630,广州,中山大学附属第三医院感染病科）
参考文献：
[1]林潮双,王向阳,高志良.乙型肝炎疫苗免疫接种现状及对无(弱)应答的探索.国际内科学杂志,2008,35:46-49.
[2]Lin C,Zhu J,Zheng Y,et al.Effect of GM-CSF in combination with hepati上一页 [1] [2] [3] 下一页
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