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纤维蛋白原基因启动子区Hae 多态性和血浆纤维蛋白原浓度与冠心病的相关性研究 |
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马会利 陈纪林
【摘要】 目的 研究β纤维蛋白原基因启动子区HaeⅢ多态性(H1H1、H1H2、H2H2)和血浆纤维蛋白原浓度与冠心病（CHD）之间的关系。方法 对66例CHD患者和53例健康对照者分别进行β HaeⅢ多态性分析和血浆纤维蛋白原浓度测定。抽提DNA采用酚、氯仿方法，血浆纤维蛋白原浓度测定采用Follin酚法。结果 （1）CHD患者携带H1H2，H2H2基因型频率较正常对照组明显增高（P<0.01）；（2）β HaeⅢ多态性与血浆纤维蛋白原浓度间存在显著正相关（r=0.65,P<0.001），以β HaeⅢ酶切位点缺失的H1H2、H2H2基因型血浆纤维蛋白原浓度明显增高（P<0.01）。结论 CHD患者携带H1H2，H2H2基因型频率高，血浆纤维蛋白原浓度高，是CHD患者动脉血栓形成的主要原因之一。
【关键词】 冠状动脉疾病 β HaeⅢ多态性 纤维蛋白原
The association between β HaeⅢ polymorphisms located in
the promoter region of β fibrinogen gene, plasma
fibrinogen levels and coronary heart disease
MA Huili CHEN Jilin
Research Laboratory of CHD, Cardiovascular Institute and Fu Wai Hospital, Beijing 100037
【Abstract】 Objective To study the association between plasma fibrinogen levels and the β HaeⅢ polymorphisms located in the promoter rigion of the β fibrinogen gene in patients with coronary heart disease (CHD). Methods β HaeⅢ polymorphisms and plasma fibrinogen levels were simultaneously measured in 66 patients with CHD and 53 control subjects. DNA was extracted using the phenol/chloroform method. Plasma fibrinogen concentration was assayed with Follin phenol method. Results (1) The frequencies of the genotype H1H2, H2H2 were higher in patients with CHD than in control subjects (P<0.01). (2) β HaeⅢ polymorphisms are significantly associated with plasma fibrinogen levels (r=0.65, P<0.001). The genotypes (H1H2,H2H2) which lost cutting site for β HaeⅢ enzyme showed that plasma fibrinogen levels were higher in this group (P<0.01).Conclusion The frequencies of the genotype H1H2, H2H2 were higher in patients with CHD as compared with control subjects and plasma fibrinogen levels in CHD patients were significantly increased. It may be one of the major factors of thrombosis in patients with coronary heart disease.
【Key words】 coronary heart disease β[1] [2] 下一页
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