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中国人群2型糖尿病患者付氧酶基因多态性与冠心病关系的研究 |
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尤蓓 于金德 陆林 乐玮 陶蓉 何汝敏 蔡煦 朱鼎良 龚兰生
摘 要：目的 探讨中国2型糖尿病患者付氧酶(PON)基因与并发冠心病(CAD)的关系。方法利用聚合酶链反应—变性梯度凝胶电泳技术对49例2型糖尿病合并CAD患者、49例未合并CAD的2型糖尿病对照者和101例健康对照者进行PON基因外显子筛查。结果 发现中国人群PON基因第191位密码子存在Gln191-Arg多态性，等位基因以A/B表示。CAD患者PON基因的3种基因型(AA、AB和BB)的构成比与2型糖尿病对照和健康对照差异有显著性，B等位基因频率较两对照组显著增高(0.79比0.62和0.61，均P＜0.01)。结论 中国2型糖尿病患者PON基因第191位密码子的Gln191-Arg多态性与并发CAD有关，B等位基因是2型糖尿病并发CAD的危险因素之一。
关键词：糖尿病，非胰岛素依赖型； 冠状动脉疾病； 聚合酶链反应； 付氧酶； 变性梯度凝胶电泳
The relationship between paraoxonase gene polymorphism and coronary artery disease in type 2 diabetes mellitus in a Chinese population
YOU Bei YU Jinde LU Lin et al
（Department of cardiology,Ruijin Hospital,Shanghai Second Medical University,Shanghai 200025）
Abstract：Objective To ascertain the relationship between paraoxonase (PON) gene and coronary artery disease (CAD) morbidity in Chinese type 2 diabetes mellitus (type 2 DM) patients. Methods The exons of PON gene were screened by polymerase chain reaction-denaturing gradient gel electrophoresis in 49 type 2 DM patients complicated with CAD, 49 CAD-uncomplicated type 2 DM as well as 101 healthy control subjects of Chinese population. Results Gln191-Arg polymorphism of the PON gene was detected in Chinese with the A/B allele frequencies 0.39 and 0.61 respectively. The genotype distribution (AA, AB and BB) of the PON gene polymorphism was significantly different between type 2 DM patients complicated with CAD and controls (type 2 DM & healthy subjects), the former had a significantly higher B allele frequency (0.79 vs 0.62 and 0.61, P＜0.01). Conclusion Gln191-Arg polymorphism of the PON gene is associated with CAD morbidity in Chinese type 2 DM patients and B allele might be a risk factor.
Key words：Diabetes mellitus, non-insulin dependent; Coronary disease; Polymerase chain reaction; Paraoxonase; Denaturing gradient gel electrophoresis▲
2型糖尿病和冠心病(CAD)均是多基因遗传性疾病，2型糖尿病又是CAD发病的独立危险因素。2型糖尿病患者大多伴随脂代谢异常，这一病理基[1] [2] [3] 下一页
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