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T细胞免疫核糖核酸治疗大鼠佐剂关节炎的研究 |
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李舒帆 赵丽娟 杨娉婷
【摘 要】 目的 探讨T细胞免疫核糖核酸(iRNA)是否可以通过调节机体免疫功能而影响关节炎病理生理过程，为类风湿关节炎治疗探索一条新路。方法 利用大鼠佐剂性关节炎(AA)动物模型，腹腔注射T细胞免疫核糖核酸，观察关节体积、关节指数及关节病理变化。结果 注射10 d后，关节体积、关节指数与关节炎对照组比较差异均有显著性(P＜0.05)，病理结果显示滑膜组织增生及炎症细胞浸润均较关节炎对照组明显减轻。结论 T细胞iRNA调节免疫系统功能抑制关节炎病理生理过程，其机制可能是iRNA通过诱生大量细胞因子的作用。
【关键词】 关节炎，佐剂性； T淋巴细胞； 免疫核糖核酸； 关节体积； 关节指数
T lymphocyte immunized RNA inhibits adjuvant arthritis in rats
LI Shufan,ZHAO Lijuan,YANG Pingting.
Department of Rheumatology,First College of China Medical University,Shenyang 110001,China
【Abstract】 Objective To study the effect of T lymphocyte immunized RNA (iRNA) in adjuvant arthritis (AA) in rats.Methods Arthritis was produced in Wistar rats after single injection of Freund′s complete adjuvant (FCA).After 14 days injection,the rats which had arthritis were given intraperitoneal injection of iRNA.Then the severity of arthritis was inspected by surveying the volume of paw and the arthritic index.Results The group treated with iRNA developed milder arthritis than the arthritic rats without it 10 days later (P＜0.05),and the difference was more significant 15 days later (P＜0.01).The arthritic index of the rat treated with iRNA was (8.6±1.9),and that of the other arthritic group was (10.6±2.1).The difference between the two groups was significant (P＜0.05).Histopathology showed that the less synovial hyperplasia and the fewer infiltration of inflammatory cells in the group treated with iRNA than without it.Conclusion Administration of iRNA can inhibit the development of AA.
【Key words】 Arthritis,adjuvant; T lympbocytes; Immunized RNA; Arthritic volume; Arthritic index
类风湿关节炎(RA)是一种病因未明的常见的自身免疫性疾病，其基本病理为关节滑膜慢性炎症。用完全福氏佐剂免疫Wistar大鼠诱发的佐剂关节(AA)，表现出多关节炎、关节畸形及在组织病理学上的改变与RA极为相似［1］。利用免疫核糖核酸(iRNA)治疗重症细菌感染及肿瘤已有诸多报道，iRNA是否通过调节机体免疫功能而影响关节炎病理生理过程值得探讨。本实验首次观察了T细胞iRNA对AA大鼠的影响，现报道如下。
1 材料与方法
1.1 实验动物及主要材料
1.1.1 动物：Wistar大鼠，购于沈阳市药品检验所。
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