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实验性癫痫与 氨基丁酸和谷氨酸脱羧酶的关系 |
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f rats was induced by daily injection of picrotoxin (PTX) intraperitoneally. Results The average value of temporal GABA content (3.82±0.81 μmol/mg) and GAD activity (11.85±2.56 μmol/mg) in Group B was much higher than that in Group A (P＜0.05). In comparison with Group B, the temporal GABA content (3.27±1.42 μmol/mg) and GAD activity (8.91±2.44 μmol/mg) in Group C were decreased, but higher than that in Group A. The results of GABA content and GAD activity between Group A and Group D revealed no significant difference.Conclusions The changes of GABA and GAD were closely related to the epileptic seizure. The results suggest that the augmented GABAergic neurotransmission is the selfprotective and anticonvulsive mechanism in this model. Phenobarbital sodium can protect it from seizure provided that the SD rats were given daily injection of PTX intraperitoneally.
【Key words】 Epilepsy； GABA； Chromatography, high pressure liquid; Glutamate decarboxylase
癫痫是神经内科的常见病，严重影响患者的工作能力和生活质量。癫痫发作是由于大脑神经元异常放电所致，其发生机制仍不十分清楚。有关γ-氨基丁酸（GABA）浓度和谷氨酸脱羧酶（GAD）活性在癫痫中变化的报道存在矛盾，这些变化是癫痫发生的原因或结果不能肯定。我们采用印防己毒(PTX)腹腔注射，制做SD鼠癫痫点燃模型，通过检测癫痫发作期和间歇期脑组织GABA浓度和GAD活性来探讨其与癫痫发生的关系，并用药物干预癫痫的发作来探讨药物对上述指标的影响。
材料与方法
1. 实验动物： 健康SD鼠（由湖南医科大学实验动物中心提供）28只，6～8周龄，体重140～180&n上一页 [1] [2] [3] [4] [5] [6] 下一页
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