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胰淀素对大鼠胰岛素分泌的影响及其机理的探讨 |
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朱铁虹 尹潍 张卫华 高淑伟 畅继武
【摘要】 目的 为研究胰淀素(Amylin)对大鼠胰岛细胞分泌胰岛素的影响和机理。方法 用放免和荧光方法分别检测经不同浓度胰淀素温育的大鼠胰岛细胞的胰岛素分泌量、细胞内Ca++及cAMP含量。结果 在胰淀素10μmol/L组中高糖刺激下的胰岛素含量、细胞内Ca++含量，细胞内cAMP含量分别为0.89±0.21ng/ml、63±10nmol/L、29.3±3.3pmol/1×106细胞与对照组(1.75±0.42ng/ml、135±10nmol/L、36.3±7.4pmol/L)比较，其中胰岛素分泌量、细胞内Ca++含量均明显降低，P＜0.01，细胞内cAMP含量有减少趋势。结论 高浓度的胰淀素抑制了胰岛细胞内Ca++升高和/或细胞内cAMP形成，可能是胰岛素分泌减少的原因之一。
【关键词】 胰淀素 胰岛素 钙离子 环磷酸腺苷
Effect of amylin on insulin secretion in rat pancreatic islets and its mechanism Zhu Tiehong,Yin Wei,Zhang Weihua,et al.Department of Endocrinology,The General Hospital,Tianjin Medical University,Tianjin,300052
【Abstract】 Objective To study the effect of Amylin on insulin secretion in rat pancreatic islet cells and its mechanism. Methods Radio-immunological and fluorescence methods were used to determine the amount of insulin secretion as well as content of intracellular Ca++ and cAMP by the rat islet cells incubated with amylin in different concentrations. Results In the 10μM/L amylin group, the glucose-stimulated insulin release volume, intracellular Ca++ and cAMP content were 0.89±0.21ng/ml, 63±10nmol/L and 29.3±3.3pmol/1×106 cell respectively as compared with the control group (1.75±0.41ng/ml, 135±10nmol/L and 36.3±7.4 pmol/1×106 cells respectively). The insulin release volume and intracellular Ca++decreased remarkably (P＜0.01), while cAMP content had a tendency to decrease. Conclusion High concentration of amylin is responsible for the prevention of rise of Ca++ inside the islet B-cells and /or the decrease of cAMP content which is one of the causes that bring about the decreased release of insulin.
【Key words】 Amylin Insulin Ca++ cAMP
(Chin J Endocrinol Metab, 1998, 14:186-189)
胰淀素(Amylin)，也称胰岛淀粉样多肽(Islet Amyloid polypeptide IApp)是从胰岛淀粉样蛋白中提取的，含有37个氨基酸序列的多肽，分子量为[1] [2] [3] 下一页
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