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扩张型心肌病抗心肌 1与M2受体自身抗体的初步研究 |
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A preliminary study of autoantibodies against the myocard
ial β1 and M2 receptors in patients with dilated cardiomyopathy
张麟 刘慧荣 赵荣瑞 张健 惠汝太 刘力生
Zhang Lin, Liu Huirong, Zhao Rongrui, et al
【摘要】 目的 研究抗β1与抗M2受体的自身抗体是否与扩张型心肌病(DCM)有关。
方法 以心脏β1与M2受体细胞外第二环表位肽段的合成肽作为抗原，应用酶联免疫吸
附测定(ELISA)技术检测52例DCM患者和41例正常人血清中抗β1与M2受体的自身抗体。
结果 DCM患者血清中有22例(42.3%)与29例(55.8%)的抗β1与抗M2受体的自身抗体，均
显著高于正常人血清中仅5例(12.2%)与4例(9.8%)相应抗体的检出(P＜0.01)；DCM患者
血清中抗β1与M2受体的自身抗体滴度分别为1∶103与1∶128，远高于正常人1∶40与
1∶24的相应抗体滴度(P＜0.01)； 心功能Ⅱ～Ⅲ级的阳性率是心功能Ⅳ级的2倍以上
且抗体滴度大多在1∶160～1∶2560，而心功能Ⅳ级的抗体滴度全部在1∶20～1∶80。
患者血清中抗β1与抗M2受体自身抗体的出现呈显著正相关(r=0.96)。结论 DCM患者
不仅存在抗心肌β1和M2的自身抗体而且他们的抗体滴度也明显高于正常对照组。
【关键词】 心肌病，充血性 受体，肾上腺素能β1 受体，胆碱能 自身抗体
A preliminary study of autoantibodies against the myocardial β1 and M2 receptors in patients
with dilated cardiomyopathy Zhang Lin, Liu Huirong, Zhao Rongrui, et al. Physiology Department for
Circulation Research, Medical University of Shanxi, Taiyuan 030001； Fu Wai Hospital and Cardiovascular
Institute, CAMS and PUMC, Beijing 100037
【Abstract】 Objective To determine whether the autoantibodies against β1-adrenergic and
M2-muscarinic receptors are related to patients with dilated cardiomyopathy (DCM). Methods
Two synthetic peptides corresponding to amino acid sequences 197-222 and 169-193 of the
second extracellular loops of the β1-adrenergic and M2 muscarinic receptors respectively were
used as antigens to screen sera from patients with DCM (n=52) and healthy blood donors (NC,n=41)
as controls. Results By ELISA, the sera from 22 patients of DCM (42.3%), 5 of NC (12.2%) (P＜0.05)
recognized the β1-adrenergic receptor peptide at dilution varying from 1∶20 to 1∶2560. The
positive rates of autoantibodies against β1 and M2 receptors of cardiac function class Ⅱ[1] [2] [3] 下一页
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