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氨基胍对糖尿病大鼠肾组织中糖化终产物受体mRNA表达的调节 |
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黄宇峰 林善锬 程梅芬 周江华
【摘要】 目的 研究氨基胍对糖尿病肾组织内糖化终产物(AGEs)受体(RAGE)mRNA表达的影响。方法 采用逆转录聚合酶链式反应(RT-PCR)检测病程8周肾内RAGE mRNA水平。结果诱发糖尿病4周后糖尿病大鼠肾皮、髓质内RAGE mRNA表达增加(P＜0.05)，8周时更为明显(P＜0.05)；口服补充氨基胍4周对肾内这种改变未产生影响(P＞0.05)，给药8周，糖尿病大鼠肾皮、髓质内增强的RAGE mRNA表达明显缓解(P＜0.05)。与此同时，氨基胍在不影响血糖的基础上，使糖尿病大鼠升高的糖化血红蛋白(GHb)水平下降26.72%。结论 糖尿病状态下，肾组织内RAGE mRNA高表达可能是高血糖诱发AGEs形成的结果；氨基胍可通过调节RAGE mRNA的异常表达，缓解AGEs对肾组织的损伤。
【关键词】 氨基胍 糖尿病 肾组织 糖化终产物受体 信使RNA
Effect of aminoguanidine on mRNA expression of specific receptor of the advanced glycation end products in diabetic kidney tissue in rats
HUANG Yufeng,LIN Shanyan,CHENG Meifen,et al
.Department of Nephrology,Huashan Hospital,Shanghai Medical University,Shanghai,200040
【Abstract】Objective Investigating the effect of aminoguanidine (AG) on RAGE (receptor for advanced glycation end products) mRNA expression in renal tissue of streptozotocin (STZ)-induced diabetic rats. Methods Four groups of rats, including (1)control rats without AG； (2)control rats with AG； (3)diabetic rats without AG and (4)diabetic rats receiving AG, were observed for 8 weeks, with the measurements of RAGE mRNA expression every 4 weeks by quantitative reverse transcription polymerase chain reaction (RT-PCR). Results After 4 weeks of diabetes inducement,RAGE mRNA levels showed a continuous increase until 8th week, in both diabetic renal cortex and medulla (P＜0.05, diabetes vs control). The RAGE mRNA levels in renal cortex and medulla of control rats did not change significantly with age. AG, as the prototype inhibitor of AGEs formation, when administered to diabetic rats for 4 weeks, did not have any visible effect on the alterations of renal RAGE mRNA in diabetes. By continuous administration up to 8th week, those alterations of RAGE mRNA both in diabetic renal cortex and medulla were all attenuated (P＜0.05, diabetes with AG vs diabetes without AG). Similarly, after 8 weeks, diabetic rats had a significantly high[1] [2] [3] 下一页
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