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重型丙型肝炎肝外组织丙型肝炎病毒感染状态免疫组化研究 |
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严福明 郝飞 顾长海 赵利斌 陈安慎 赵西平 郝连杰
【摘要】 目的 探讨丙型肝炎患者肝外组织丙型肝炎病毒(HCV)感染状态、致病性及其意义。方法 采用免疫组化法检测9例重型丙型肝炎患者肾、心、胰腺和肠等肝外组织内HCV多种抗原表达，并观察各组织病理改变。结果 9例中6例(66.7%)在肝外组织检出HCV抗原。抗原定位于细胞浆内，呈均质型、包涵体型和膜下型。组织内抗原表达阳性细胞数量较少，不同组织间阳性细胞种类、数量和分布略有差异。病理学观察未发现细胞内HCV抗原阳性表达与组织病变有明确相关性。结论 HCV可以感染肝外组织，但未显示显著致病性。作为致病因子的HCV持续存在肝外多种脏器细胞内，在丙型肝炎慢性化和干扰素治疗后病情复发中可能起重要作用。
【关键词】 肝炎病毒，丙型 抗原表达 免疫组织化学
Immunohistochemical study on the state of extrahepatic hepatitis C virus infection in severe hepatitis C
YAN Fuming, HAO Fei, GU Changhai, et al.
Department of Infectious Diseases, The 161st Central Hospital of Chinese PLA, Wuhan 430010
【Abstract】 Objective To explore the state of extrahepatic hepatitis C virus (HCV) infection, viral pathogenicity and its significance in hepatitis C patients.Methods Immunohistochemical assay was used to examine the expression of various HCV antigens in the kidney, heart, pancreas and intestine from 9 severe hepatitis C patients. Relationship between the expression of HCV antigens and tissue lesions was evaluated.Results HCV antigens in extrahepatic tissues were detected in 6 of 9 (66.7%) hepatitis C patients and the expression of HCV antigens was observed only within cytoplasm in the style of homogenous, inclusion and submembrane distribution. The amount of positive-staining cells was less in the tissue and there were a few differences of kind, amount and distribution of HCV antigens-positive-staining cells among different extrahepatic tissues. There was no relationship between the expression of HCV antigens and tissue lesion.Conclusions These results show that HCV exists in different extrahepatic tissues, but do not bring out obvious cell injury. As a etiologic agent, the persistence of HCV in extrahepatic tissues may take an important role in the relapse as well as the chronicity of hepatitis C after discontinuation of interferon therapy.
【Key words】 Hepatitis C virus Antigen expressi[1] [2] 下一页
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