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类风湿关节炎滑膜巨噬细胞来源的研究 |
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戴冽 叶志强 汤美安 尹培达 苏祖兰
摘 要:目的 了解类风湿关节炎(RA)滑膜巨噬细胞的来源。方法 采用免疫组化LSAB法对26例RA滑膜,20例非RA滑膜及7例正常滑膜进行连续切片CD68和PCNA染色。结果 正常滑膜衬里层细胞几乎全部CD68阳性而PCNA阴性,衬里下层CD68和PCNA阳性细胞极少;RA滑膜衬里层和衬里下层PCNA阳性细胞数较非RA滑膜明显增多(P<0.05),衬里层滑膜细胞大部分CD68阳性,衬里下层CD68阳性细胞数较非RA滑膜明显增多(P<0.05),并且衬里层和衬里下层部分CD68阳性的细胞PCNA也阳性。结论 RA滑膜增多的A型巨噬样滑膜细胞和衬里下层的巨噬细胞都有局部增生,表明RA滑膜衬里下层聚集的巨噬细胞除来源于血液中的单核细胞趋化转移外,还与巨噬细胞在炎症局部增生有关。 关键词:关节炎,类风湿; 巨噬细胞; 滑膜
Research on source of macrophage in rheumatoid synovium
DAI Lie YE Zhiqiang TANG Meian et al (Department of Rheumatic Immunology,Third Affiliated Hospital of Sun Yat-sen University of Medical Sciences,Guangzhou 510630,China)
Abstract:Objective To know the source of macrophage in rheumatoid synovium.Methods CD68 and PCNA were detected by immunohistochemical LSAB method on serial synovial sections of 26 cases of RA,20 cases of control non-RA and 7 cases of normal synovia.Results The lining cells of normal synovia were almost all CD68-positive and PCNA-negative while CD68-and PCNA-positive cells in sublining area were scarce;PCNA-positive cells in lining and sublining areas of RA synovia were significantly increased (P<0.05),most lining synoviocytes were CD68-positive and the CD68-positive cells in sublining area were also significantly increased (P<0.05),some CD68-positive cells in lining and sublining areas of RA synovia were also PCNA-positive.Conclusion Both macrophage-like synoviocytes and infiltrated macrophages in sublining area of RA synovia have local proliferation. Key words:Arthritis,rheumatoid; Macrophage; Synovial membrane▲
巨噬细胞在类风湿关节炎(RA)关节破坏中的作用及重要性的认识是近年RA发病机制研究的一大进展。RA滑膜中有大量聚集的巨噬细胞,但巨噬细胞在RA滑膜中的来源及聚集机制尚未完全阐明。为此,我们采用滑膜组织连续切片CD68和增生细胞核抗原(PCNA)免疫组化染色方法,探讨巨噬细胞局部增生在RA滑膜巨噬细胞聚集中的作用。
1 材料与方法
1.1 滑膜标本来源及对象:所有滑膜标本均取自关节镜手术及关节置换术患者,其中RA滑膜26例,诊断符合1987年美国风湿病学会(ARA)修订的诊断标准[1],男8例,女18例,年龄21~72岁,平均年龄(46±13)岁,病程0.5~26年,平均病程(9±7)年;非RA组骨关节炎(OA)滑膜14例,男6例,女8例,年龄39~75[1] [2] 下一页 上一个医学论文: 参麦注射液对骨关节炎模型动物关节液中白细胞介素 下一个医学论文: 高血压对狼疮肾炎预后的影响
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