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干扰素对人甲状腺细胞表面抗原表达的影响 |
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尤欣 滕卫平 单忠艳
【摘要】 目的 研究干扰素(IFN-α、IFN-γ)及激素对人正常甲状腺细胞表面抗原表达的影响，以探讨IFN-α导致自身免疫性甲状腺病(AITD)的可能机制。方法 应用IFN-α、IFN-γ、促甲状腺激素(TSH)及催乳素(PRL)，刺激体外培养的来自6名正常人的甲状腺细胞，通过免疫荧光染色及流式细胞仪测定其表面抗原—HLA-DR、细胞间粘附分子-1(ICAM-1)、B 7.1和甲状腺过氧化物酶(TPO)的表达。结果 (1)IFN-α明显诱导甲状腺细胞表达ICAM-1、B 7.1和TPO(分别为P＜0.01、P＜0.01、P＜0.05)；(2)IFN-γ明显诱导甲状腺细胞表达HLA-DR、ICAM-1，但对B 7.1的表达无促进作用(P＜0.01、P＜0.01、P＞0.05)；(3)PRL显著诱导甲状腺细胞表达ICAM-1、B 7.1和TPO(P＜0.05、P＜0.01、P＜0.01)。结论 协同刺激信号对AITD的发生起着关键作用。IFN-α显著诱导甲状腺细胞表达协同刺激信号(B 7.1)和自身抗原TPO，可能是临床应用IFN-α诱致AITD的致病原因之一。PRL在产后甲状腺炎发病和发展中的作用应予以关注。
【关键词】 干扰素 甲状腺细胞 自身免疫 协同刺激信号 甲状腺过氧化物酶
Effect of interferon on surface antigen expression of human thyrocytes
YOU Xin,TENG Weiping,SHAN Zhongyan.
Department of Endocrinology, The First Affiliated Hospital of China Medical University,Shenyang,110001
【Abstract】 Objective To study the effects of interferons (IFN-α,IFN-γ) on the surface antigen expression of human thyrocytes. Methods The expressions of HLA-DR, ICAM-1, B 7.1 and thyroid peroxidase (TPO) by cultured thyrocytes from six normal persons induced by IFN-α, IFN-γ, TSH and PRL were investigated using immunofluorescence staining with flow cytometer. Results (1)IFN-α significantly stimulated the expression of ICAM-1, B 7.1 and TPO, as compared with those of control group (P＜0.01, ＜0.01, ＜0.05， respectively). (2)IFN-γ markedly enhanced the expression of HLA-DR and ICAM-1, but not B 7.1 (P＜0.01, ＜0.01, ＞0.05, respectively). (3)PRL increased expression of ICAM-1, B 7.1, as well as overexpression of TPO (P＜0.05, ＜0.01, ＜0.01, respectively). Conclusion The thyroid autoimmunity induced by IFN-α is associated with the expression of ICAM-1, B 7.1 and TPO. IFN-γ could not induce the expression of B 7.1, therefore it is not an initiator in AITD. In addition, more attention should be paid to PRL which possibly plays an i[1] [2] 下一页
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