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瘦素对肝细胞葡萄糖氧化及葡萄糖激酶基因表达的影响 |
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曹筱佩 明文玉 程桦 杨荣泽 傅祖植
【摘要】 目的 观察瘦素(Leptin)对肝细胞葡萄糖氧化的影响及其剂量效应，并观察其对葡萄糖激酶基因表达的影响。方法 正常大鼠肝细胞，以不加Leptin与加不同剂量的Leptin培养1小时后，以液体闪烁计数法检测肝细胞氧化〔U-14C〕葡萄糖的量。以RT-PCR法检测Leptin 100μg/L处理组与对照组肝细胞葡萄糖激酶(GK)mRNA的表达。结果 当Leptin浓度为10μg/L时，肝细胞葡萄糖氧化与对照组无明显差异，当Leptin浓度上升为50μg/L后，葡萄糖氧化较对照组显著下降(P＜0.05)，并随剂量增大抑制作用加强。Leptin 100μg/L处理组GK mRNA表达水平(以β-actin校正)亦显著低于对照组(0.43±0.07比0.87±0.09，P＜0.01)。结论 低剂量瘦素对肝细胞葡萄糖氧化无明显影响，高浓度瘦素则具剂量依赖性抑制作用，并至少部分通过抑制GK基因表达而达到。
【关键词】 肝细胞 葡萄糖氧化 葡萄糖激酶 瘦素
Effects of leptin on glucose oxidation and glucokinase gene expression in cultured liver cells
CAO Xiaopei,MING Wenyu,CHENG Hua,et al.
Department of Endocrinology,Memorial Hospital,Sun Yat-sen University of Medical Sciences, Guangzhou,510120
【Abstract】 Objective To observe the effects of leptin on glucose oxidation and glucokinase gene expression in rat liver cells. Methods Rat liver cells were incubated with leptin of different doses (ranging from 10μg/L to 200μg/L). Control liver cells were incubated without leptin. 1h later, glucose oxidation was determined by liquid scintillation counting. In the control group and 100μg/L leptin-treated group, RT-PCR was used to determine glucokinase (GK) gene expression (corrected by β-actin). Results Comparing with the control group, treatment with leptin of 10μg/L for 1h had no significant effect on glucose oxidation in liver cells. However, at the dose of 50μg/L, leptin significantly inhibited glucose oxidation (1.78±0.59 vs 3.69±1.08, P＜0.05). This inhibitory effect of leptin was dose dependent. At the dose of 200μg/L, glucose oxidation decreased to 1.60±0.18 (P＜0.01). The glucokinase mRNA expression (corrected by β-actin) also reduced significantly in 100μg/L leptin-treated group than in the ocntrol group (0.43±0.07, vs 0.87±0.09, P＜0.01). Conclusion Physiological dose of leptin has little effect on [1] [2] [3] 下一页
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