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雄激素致不孕大鼠肥胖 |
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孙斐 俞瑾 笪翠娣 黄娅林
【摘要】 目的 探讨9日龄雄激素致不孕大鼠(9d-ASR)肥胖-无排卵机制及滋肾阴药的作用。方法 给出生9日龄SD雌性大鼠一次性皮下注射丙酸睾丸酮，建立9d-ASR模型。检测雌激素受体(ER)和神经肽Y(NPY)神经元共存情况；检测9d-ASR下丘脑ER、NPY及促性腺激素释放激素(GnRH)含量变化；测定血雌二醇(E2)、睾酮(T)、瘦素、促卵泡激素(FSH)、促黄体激素(LH)的变化。结果 ASR呈能量失衡致肥胖状态，血E2、T、瘦素水平明显增加(P＜0.01)，FSH、LH明显低下(P＜0.01)，下丘脑ER存在于NPY神经元上，其数目减少，NPY表达增强，GnRH分泌下降。NPY与ER或GnRH均呈负相关(r=-0.7104,P＜0.01；r=-0.8802,P＜0.01)。瘦素与体重呈正相关(r=0.8977,P＜0.01)，与FSH、LH呈负相关(r=-0.7517,P＜0.01；r=-0.8444，P＜0.01)。用滋肾阴药治疗后，各神经内分泌-代谢指标均恢复正常。结论 9d-ASR模型有神经内分泌-代谢失调而致肥胖并引起低促性腺激素性性腺功能低下性无排卵，滋肾阴中药有纠正这些失调而产生减肥及促排卵的作用。
【关键词】 雄激素 大鼠 雌激素受体 神经肽Y 瘦素 中药
Mechanism of obesity-anovulation in androgen-sterilized rat and the weight reducing and ovulation inducing effects of tonifying Kidney yin herbs
SUN Fei,YU Jin,DA Cuidi,et al
.Obstetric and Gynecologic Hospital,Shanghai Medical University,Shanghai,200011
【Abstract】 Objective To explore the mechanism of obesity and chronic anovulation in androgen-sterilized rat at age of 9 days (9d-ASR) and the effect of herbs of tonifying Kidney yin. Methods 9d-ASR model was established by injecting testosterone propionate subcutaneously to SD female rat at age of 9 days. Double immunofluorescent staining was carried out on sections through arcuate nucleus (ARC) to determine whether neuropeptide Y (NPY)-containing neurons expressed estrogen receptors (ER). The immunoreactive amounts of ER, NPY neurons and gonadotropin-releasing hormone (GnRH) fibers of hypothalamic ARC-median eminence (ME) in 9d-ASR were studied with immunohistochemistry. Serum estrogen (E2), testosterone (T), leptin, follicular stimulating hormone (FSH) and luteinizing hormone (LH) were measured with radioimmunoassay. All parameters were reevaluated after administration of herbs to 9d-ASR. Results Estrogen receptors were predominantly distributed in the cytoplasm of NPY-containing neurons as well as in the nucleus and axons of NPY-synthesizing neurons. Compared with normal group, [1] [2] [3] 下一页
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