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完全弗氏佐剂预防NOD鼠胰岛炎和糖尿病的机理研究 |
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杨竹林 伍汉文 周智广 廖二元 杨文
【摘要】 目的 研究Bax蛋白和热休克蛋白(HSP)70在完全弗氏佐剂(CFA)预防NOD鼠胰岛炎和糖尿病中作用及意义。方法 给4周龄雌性NOD鼠后脚板注射50μl CFA和生理盐水(NS组)，于30周龄或糖尿病发生时处死，胰腺组织置10%中性福尔马林固定后常规制成石蜡切片，HE染色后行光镜下胰岛炎评分，ABC免疫组化染色Bax蛋白和HSP70并光镜下计数。结果 CFA组鼠胰岛内炎计数和胰岛炎评分均数均明显低于NS组鼠(P＜0.01)；CFA组鼠无1只发生糖尿病，NS组鼠中有3只(60%)发生糖尿病；CFA组鼠胰岛细胞Bax蛋白阳性率明显低于NS组鼠，而HSP70阳性率明显高于NS组鼠，均有极显著性差异(P＜0.01)；胰岛炎分值与Bax蛋白阳性率呈正相关(r=0.613)，与HSP70阳性率呈负相关(r=-0.913)。结论 CFA能减轻NOD鼠胰岛炎发生程度和预防糖尿病发生，CFA能抑制胰岛细胞Bax蛋白表达和诱导胰岛细胞HSP70表达。
【关键词】 完全弗氏佐剂 胰岛炎 糖尿病 非肥胖糖尿病鼠 癌基因蛋白类 热休克蛋白类
Prevention of insulitis and diabetes by complete Freund s adjuvant in NOD mice
YANG Zhulin,WU Hanwen,ZHOU Zhiguang,et al
Research Laboratory of Hepatobiliary Disease,The Second Affiliated Hospital,Hunan Medical University,Changsha,410011
【Abstract】 Objective To study the effects and significance of Bax protein and heat shock protein 70 (HSP70) in prevention of insulitis and diabetes by complete Freund s adjuvant (CFA) in female NOD mice. Methods 5 female NOD mice of CFA-treated group were injected into the pad of hind foot with 50 μl CFA and 5 female NOD mice of control group with 50μl normal saline (NS) at 4 weeks of age. They were sacrificed at 30th week of age or when diabetes occurred. The pancreatic tissues were routinely processed and HE-stained and scoring of insulitis was done under optical microscopy. The Bax oncoprotein and HSP70 were stained with ABC immunohistochemical method and the counts of positive cells in islets were finished under high power field. Results The incidence of in-insulitis and the average score of insulitis were significantly lower in CFA-treated mice than that in NS-treated mice (P＜0.01). None of CFA-treated mice and 3 out of 5 NS-treated mice developed diabetes. The positive rate of Bax oncoprotein in islet cells was significantly lower in CFA-treated mice than that in NS-treated mice, the positive rate of HSP70 in islet cells was significantly higher in CFA-t[1] [2] [3] 下一页
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